Cloned (Comment) | Organism |
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- |
Pyrococcus furiosus |
Crystallization (Comment) | Organism |
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hanging-drop vapour diffusion method, crystals of the archaeal DNA ligase from Pyrococcus furiosus aree obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 A resolution. They belong to the monoclinic space group P2(1), with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 A, beta = 108.9°. The asymmetric unit contains one ligase molecule | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
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Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
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- |
Pyrococcus furiosus |
General Information | Comment | Organism |
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physiological function | DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase | Pyrococcus furiosus |