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Literature summary for 6.5.1.1 extracted from

  • Nishida, H.; Tsuchiya, D.; Ishino, Y.; Morikawa, K.
    Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus (2005), Acta Crystallogr. Sect. F, 61, 1100-1102.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method, crystals of the archaeal DNA ligase from Pyrococcus furiosus aree obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 A resolution. They belong to the monoclinic space group P2(1), with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 A, beta = 108.9°. The asymmetric unit contains one ligase molecule Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
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-
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Purification (Commentary)

Purification (Comment) Organism
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Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase Pyrococcus furiosus