Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.1 extracted from

  • Cardona-Felix, C.S.; Pastor-Palacios, G.; Cardenas, H.; Azuara-Liceaga, E.; Brieba, L.G.
    Biochemical characterization of the DNA ligase I from Entamoeba histolytica (2010), Mol. Biochem. Parasitol., 174, 26-35.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Start cells Entamoeba histolytica

Protein Variants

Protein Variants Comment Organism
K326 catalytically inactive Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
NaCl complete inhibition at above 200 mM Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000064
-
ATP in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Entamoeba histolytica 5737
-
nucleus a population of the DNAligI protein is translocated from the cytoplasm into the nuclei Entamoeba histolytica 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ DNAligI uses Mn2+ or Mg2+ as metal cofactors, optimal ligation activity at 4 mM of Mg2+ Entamoeba histolytica
Mn2+ DNAligI uses Mn2+ or Mg2+ as metal cofactors, optimal ligation activity at 1 mM of Mn2+ Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
x * 75000, SDS-PAGE Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica C4M5H3
-
-
Entamoeba histolytica HM1:IMSS C4M5H3
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography and phosphocellulose column chromatography Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Entamoeba histolytica AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Entamoeba histolytica HM1:IMSS AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
additional information DNAligI performs the three conserved steps of a DNA ligation reaction: adenylation, binding to a 5'-phosphorylated nicked DNA substrate and sealing of the nick. DNAligI is also able to ligate a RNA strand upstream of a nucleic acid nick, but not in the downstream or the template position Entamoeba histolytica ?
-
?
additional information DNAligI performs the three conserved steps of a DNA ligation reaction: adenylation, binding to a 5'-phosphorylated nicked DNA substrate and sealing of the nick. DNAligI is also able to ligate a RNA strand upstream of a nucleic acid nick, but not in the downstream or the template position Entamoeba histolytica HM1:IMSS ?
-
?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
DNA ligase I
-
Entamoeba histolytica
DNAligI
-
Entamoeba histolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Entamoeba histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
ATP in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Entamoeba histolytica

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 DNAligI activity gradually increases from pH 6.5 to 8.0 and displays a sharp decline at pH 8.5 Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
ATP specifically dependent on ATP Entamoeba histolytica
additional information DNAligI is not active using NAD+, UTP, CTP, GTP, dATP, and dGTP as cofactors Entamoeba histolytica

General Information

General Information Comment Organism
physiological function DNAligI is involved in sealing DNA nicks during lagging strand synthesis and may have a role in base excision repair in Entamoeba histolytica Entamoeba histolytica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
625
-
ATP in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C Entamoeba histolytica