Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Start cells | Entamoeba histolytica |
Protein Variants | Comment | Organism |
---|---|---|
K326 | catalytically inactive | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | complete inhibition at above 200 mM | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000064 | - |
ATP | in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Entamoeba histolytica | 5737 | - |
nucleus | a population of the DNAligI protein is translocated from the cytoplasm into the nuclei | Entamoeba histolytica | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | DNAligI uses Mn2+ or Mg2+ as metal cofactors, optimal ligation activity at 4 mM of Mg2+ | Entamoeba histolytica | |
Mn2+ | DNAligI uses Mn2+ or Mg2+ as metal cofactors, optimal ligation activity at 1 mM of Mn2+ | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, SDS-PAGE | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | C4M5H3 | - |
- |
Entamoeba histolytica HM1:IMSS | C4M5H3 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography and phosphocellulose column chromatography | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Entamoeba histolytica | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Entamoeba histolytica HM1:IMSS | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
additional information | DNAligI performs the three conserved steps of a DNA ligation reaction: adenylation, binding to a 5'-phosphorylated nicked DNA substrate and sealing of the nick. DNAligI is also able to ligate a RNA strand upstream of a nucleic acid nick, but not in the downstream or the template position | Entamoeba histolytica | ? | - |
? | |
additional information | DNAligI performs the three conserved steps of a DNA ligation reaction: adenylation, binding to a 5'-phosphorylated nicked DNA substrate and sealing of the nick. DNAligI is also able to ligate a RNA strand upstream of a nucleic acid nick, but not in the downstream or the template position | Entamoeba histolytica HM1:IMSS | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase I | - |
Entamoeba histolytica |
DNAligI | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
ATP | in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Entamoeba histolytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | DNAligI activity gradually increases from pH 6.5 to 8.0 and displays a sharp decline at pH 8.5 | Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | specifically dependent on ATP | Entamoeba histolytica | |
additional information | DNAligI is not active using NAD+, UTP, CTP, GTP, dATP, and dGTP as cofactors | Entamoeba histolytica |
General Information | Comment | Organism |
---|---|---|
physiological function | DNAligI is involved in sealing DNA nicks during lagging strand synthesis and may have a role in base excision repair in Entamoeba histolytica | Entamoeba histolytica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
625 | - |
ATP | in 50 mM Tris pH 7.5, 100 mM NaCl, 10 mM dithiothreitol, at 21°C | Entamoeba histolytica |