Literature summary for 6.5.1.1 extracted from

Cotner-Gohara, E.; Kim, I.K.; Tomkinson, A.E.; Ellenberger, T.
Two DNA-binding and nick recognition modules in human DNA ligase III (2008), J. Biol. Chem., 283, 10764-10772.

Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of the zinc finger does not significantly change LigIII DNA binding affinity, nor does it abrogate the specificity of the enzyme for a nicked substrate	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Na+	in 80 mM NaCl LigIII binds non-specifically to DNA, showing equal affinity for nicked and intact DNA, in 250 mM NaCl LigIII binds specifically to nicked DNA does not bind measurably to duplex DNA	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap nickel chelating column chromatography and Sephadex S200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Homo sapiens	AMP + diphosphate + (deoxyribonucleotide)m+n	-	?

Synonyms

Synonyms	Comment	Organism
DNA ligase III	-	Homo sapiens
LigIII	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)