Literature summary for 6.5.1.1 extracted from
Smagin, V.A.; Mardanov, A.V.; Bonch-Osmolovskaya, E.A.; Ravin, N.V.
Isolation and characteristics of new thermostable DNA ligase from archaea of the genus Thermococcus (2008), Appl. Biochem. Microbiol., 44, 473-477.
No PubMed abstract available
Application
Application |
Comment |
Organism |
molecular biology |
LihTh1519 may be used for basic and applied researches in molecular biology and genetic engineering |
Thermococcus sp. |
Cloned(Commentary)
Cloned (Comment) |
Organism |
expressed in Escherichia coli strain DLT1270/pRARE |
Thermococcus sp. |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Mg2+ |
optimum concentration at 50 mM |
Thermococcus sp. |
|
Na+ |
optimum concentration at 100 mM |
Thermococcus sp. |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
63000 |
- |
SDS-PAGE |
Thermococcus sp. |
Organism
Organism |
UniProt |
Comment |
Textmining |
Thermococcus sp. |
C0LJI8 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
Ni-NTA column chromatography |
Thermococcus sp. |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m |
LigTh1519 is capable of ligating the cohesive ends and single-strand breaks in double-stranded DNA with ATP as cofactor |
Thermococcus sp. |
AMP + diphosphate + (deoxyribonucleotide)m+n |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
DNA ligase |
- |
Thermococcus sp. |
LigTh1519 |
- |
Thermococcus sp. |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
65 |
70 |
- |
Thermococcus sp. |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
50 |
75 |
the enzyme remains active at 50-75°C or higher |
Thermococcus sp. |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
80 |
- |
more than 50% Lig1519 activity is preserved after incubation of the enzyme at 80°C for 30 min |
Thermococcus sp. |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7 |
10.5 |
- |
Thermococcus sp. |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
ATP |
- |
Thermococcus sp. |
|