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Literature summary for 6.5.1.1 extracted from

  • Smagin, V.A.; Mardanov, A.V.; Bonch-Osmolovskaya, E.A.; Ravin, N.V.
    Isolation and characteristics of new thermostable DNA ligase from archaea of the genus Thermococcus (2008), Appl. Biochem. Microbiol., 44, 473-477.
No PubMed abstract available

Application

Application Comment Organism
molecular biology LihTh1519 may be used for basic and applied researches in molecular biology and genetic engineering Thermococcus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DLT1270/pRARE Thermococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum concentration at 50 mM Thermococcus sp.
Na+ optimum concentration at 100 mM Thermococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
SDS-PAGE Thermococcus sp.

Organism

Organism UniProt Comment Textmining
Thermococcus sp. C0LJI8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m LigTh1519 is capable of ligating the cohesive ends and single-strand breaks in double-stranded DNA with ATP as cofactor Thermococcus sp. AMP + diphosphate + (deoxyribonucleotide)m+n
-
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Synonyms

Synonyms Comment Organism
DNA ligase
-
Thermococcus sp.
LigTh1519
-
Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 70
-
Thermococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 the enzyme remains active at 50-75°C or higher Thermococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
more than 50% Lig1519 activity is preserved after incubation of the enzyme at 80°C for 30 min Thermococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 10.5
-
Thermococcus sp.

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermococcus sp.