Cloned (Comment) | Organism |
---|---|
gene sequence encoding FaLig is cloned into a bacterial expression vector harbouring an upstream His-tag to aid purification and expressed in Escherichia coli | Ferroplasma acidarmanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | efficient nick-joining is observed in the presence of Mg2+ and Mn2+ | Ferroplasma acidarmanus | |
Mn2+ | efficient nick-joining is observed in the presence of Mg2+ and Mn2+ | Ferroplasma acidarmanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67800 | - |
x * 67800, calculation from sequence | Ferroplasma acidarmanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ferroplasma acidarmanus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ferroplasma acidarmanus |
Subunits | Comment | Organism |
---|---|---|
? | x * 67800, calculation from sequence | Ferroplasma acidarmanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | nick-joining | Ferroplasma acidarmanus |