Literature summary for 6.5.1.1 extracted from

Cherepanov, A.V.; de Vries, S.
Kinetics and thermodynamics of nick sealing by T4 DNA ligase (2003), Eur. J. Biochem., 270, 4315-4325.

Search for more literature for 6.5.1.1

General Stability

General Stability	Organism
T4 DNA ligase gradually lose their activity when incubated at temperatures above 0°C	Tequatrovirus T4

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0006	-	ATP	-	Tequatrovirus T4

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	divalent cation required for activity, maximal activity at approx. 10 mM	Tequatrovirus T4
Mn2+	divalent cation required for activity	Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	Tequatrovirus T4	nick-ligation and blunt-end or sticky-end ligation	AMP + diphosphate + (deoxyribonucleotide)m+n	-	?

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	nick-ligation and blunt-end or sticky-end ligation	Tequatrovirus T4	AMP + diphosphate + (deoxyribonucleotide)m+n	-	?

Synonyms

Synonyms	Comment	Organism
T4 DNA ligase	-	Tequatrovirus T4

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Release 2023.1 (January 2023)