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Literature summary for 6.5.1.1 extracted from
- Purification of DNA ligase II from calf thymus and preparation of rabbit antibody against calf thymus DNA ligase II (1986), J. Biol. Chem., 261, 6888-6892.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00004 | - |
DNA | nicked DNA, enzyme DNA ligase II | Bos taurus |  |
| 0.04 | - |
ATP | DNA ligase II | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
sucrose density gradient centrifugation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DNA ligase II | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Bos taurus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
| ATP + DNA | - |
Bos taurus | AMP + diphosphate + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 68000, SDS-PAGE | Bos taurus |
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Release 2023.1 (January 2023)
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