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Literature summary for 6.5.1.1 extracted from

  • Rabin, B.A.; Chase, J.W.
    DNA ligase from Drosophila melanogaster embryos. Substrate specificity and mechanism of action (1987), J. Biol. Chem., 262, 14105-14111.
    View publication on PubMed
Search for more literature for 6.5.1.1

Inhibitors

Inhibitors Comment Organism Structure
dATP
-
 Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 ATP p(dT)20 annealed with poly(dA) Drosophila melanogaster
0.0016
-
 ATP
-
 Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DNA ligase II Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-O-(3-Thio)adenosine triphosphate + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Drosophila melanogaster 5'-O-(3-Thio)adenosine monophosphate + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m overview: activity of DNA ligase on ribo- and deoxyribopolymer substrates Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m reverse reaction: incubation of superhelical closed circular DNA molecules with the enzyme and AMP results in the production of a population of DNA molecules which have lost most of their superhelical density Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m p(dT)7U can be joined when annealed with poly(dA), joining of oligonucleotides containing a single mismatched nucleotide at their 3'-hydroxyl termini, as well as DNA containing short, complementary 5'-protruding ends, and in the presence of glycol 6000, blunt-ended duplex DNA Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m in addition to joining DNA to DNA the enzyme can join the 5'-phosphoryl terminus of RNA to the 3'-hydroxyl terminus of DNA or RNA, when they are annealed with DNA Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Drosophila melanogaster