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Literature summary for 6.5.1.1 extracted from

  • Lasko, D.D.; Tomkinson, A.E.; Lindahl, T.
    Eukaryotic DNA ligases (1990), Mutat. Res., 236, 277-287.
    View publication on PubMed
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae
-
 Schizosaccharomyces pombe
-
 Vaccinia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
 DNA nicked DNA, enzyme DNA ligase II Bos taurus
0.00011
-
 DNA nicked DNA, enzyme DNA ligase I Bos taurus
0.001
-
 ATP
-
 Drosophila melanogaster
0.002
-
 ATP DNA ligase I Bos taurus
0.002
-
 ATP ATP, ATP-diphosphate exchange reaction Bos taurus
0.04
-
 ATP DNA ligase II Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Drosophila melanogaster 5634
-
nucleus
-
 Mammalia 5634
-
nucleus DNA ligase II is more firmly retained in isolated cell nuclei than DNA ligase I Bos taurus 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 x * 85000, calculation from nucleotide sequence, SDS-PAGE Saccharomyces cerevisiae
86000
-
 x * 86000, calculation from nucleotide sequence, SDS-PAGE Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-
Drosophila melanogaster
-
 DNA ligase I and II
-
Mammalia
-
-
-
Saccharomyces cerevisiae
-
-
-
Schizosaccharomyces pombe
-
-
-
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DNA ligase I and II Mammalia

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Drosophila melanogaster
-
thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m can join a 3'-hydroxyl terminus of DNA to a 5'-hydroxyl terminus of RNA, can join oligo(rA)12*poly(dT), blunt end joining in presence of polyethylene glycol Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m inability to ligate oligo(dT)*poly(rA) Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m inability to ligate oligo(dT)*poly(rA) Mammalia AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m inability to ligate oligo(dT)*poly(rA) Saccharomyces cerevisiae AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m inability to ligate oligo(dT)*poly(rA) Bos taurus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m inability to ligate oligo(dT)*poly(rA) Schizosaccharomyces pombe AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m inability to ligate oligo(dT)*poly(rA) Vaccinia virus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + DNA
-
 Bos taurus AMP + diphosphate + ?
-
 ?

Subunits

Subunits Comment Organism
?
-
 Drosophila melanogaster
? x * 120000-130000, SDS-PAGE, gel filtration under denaturing conditions, DNA ligase I Bos taurus
? x * 85000, calculation from nucleotide sequence, SDS-PAGE Saccharomyces cerevisiae
? x * 86000, calculation from nucleotide sequence, SDS-PAGE Schizosaccharomyces pombe