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Literature summary for 6.4.1.6 extracted from

  • Dullius, C.H.; Chen, C.Y.; Schink, B.
    Nitrate-dependent degradation of acetone by Alicycliphilus and Paracoccus strains and comparison of acetone carboxylase enzymes (2011), Appl. Environ. Microbiol., 77, 6821-6825.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
three bands observed with SDS-PAGE: 85000, 78000, 20000 Da Alicycliphilus denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetone + CO2 + ATP + H2O Alicycliphilus denitrificans
-
acetoacetate + AMP + phosphate
-
?
butanone + CO2 + ATP + H2O Alicycliphilus denitrificans strain KN Bun08 also carboxylates also butanone 3-oxopentanoate + AMP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Alicycliphilus denitrificans
-
isolated from strain KN Bun08 and K601T
-

Purification (Commentary)

Purification (Comment) Organism
enriched from cell extracts of Alicycliphilus denitrificans by two subsequent DEAE Sepharose column procedures Alicycliphilus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetone + CO2 + ATP + H2O
-
Alicycliphilus denitrificans acetoacetate + AMP + phosphate
-
?
butanone + CO2 + ATP + H2O strain KN Bun08 also carboxylates also butanone Alicycliphilus denitrificans 3-oxopentanoate + AMP + phosphate
-
?

Synonyms

Synonyms Comment Organism
acetone-carboxylating enzyme
-
Alicycliphilus denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Alicycliphilus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Alicycliphilus denitrificans