Cloned (Comment) | Organism |
---|---|
expression of His-tagged MCCase in Escherichia coli strain BL21 | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | MCCase activity shows sigmoidal kinetics for all the substrates and non-Michaelis-Menten kinetics, overview | Pseudomonas aeruginosa | |
0.0008 | - |
HCO3- | pH 8.0, 30°C, recombinant methylcrotonyl-CoA carboxylase | Pseudomonas aeruginosa | |
0.0098 | - |
3-methylcrotonoyl-CoA | pH 8.0, 30°C, recombinant methylcrotonyl-CoA carboxylase | Pseudomonas aeruginosa | |
0.01 | - |
ATP | pH 8.0, 30°C, recombinant geranyl-CoA carboxylase | Pseudomonas aeruginosa | |
0.013 | - |
ATP | pH 8.0, 30°C, recombinant methylcrotonyl-CoA carboxylase | Pseudomonas aeruginosa | |
0.014 | - |
3-methylcrotonoyl-CoA | pH 8.0, 30°C, recombinant geranyl-CoA carboxylase | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonoyl-CoA + HCO3- | Pseudomonas aeruginosa | - |
ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
additional information | Pseudomonas aeruginosa | geranyl-CoA carboxylase, GCCase, may substitute for MCCase in leucine catabolism, both the MCCase and GCCase enzymes play important roles in the leucine and acyclic terpene catabolic pathways, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MCCase from Escherichia coli strain BL21 by avidin affinity chromatography | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pseudomonas aeruginosa | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
ATP + 3-methylcrotonoyl-CoA + HCO3- | substrate of MCCase, EC 6.4.1.4, and GCCAse, EC 6.4.1.5 | Pseudomonas aeruginosa | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
additional information | geranyl-CoA carboxylase, GCCase, may substitute for MCCase in leucine catabolism, both the MCCase and GCCase enzymes play important roles in the leucine and acyclic terpene catabolic pathways, overview | Pseudomonas aeruginosa | ? | - |
? | |
additional information | MCCase shows no activity with geranyl-CoA, while the geranyl-CoA carboxylase, EC 6.4.1.5, is also active with 3-methylcrotonoyl-CoA in addition to the preferred substrate geranyl-CoA | Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-methylcrotonyl coenzyme A carboxylase | - |
Pseudomonas aeruginosa |
MC-CoA carboxylase | - |
Pseudomonas aeruginosa |
MCCase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
native and recombinant enzyme | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
native and recombinant enzyme | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas aeruginosa |