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Literature summary for 6.4.1.4 extracted from

  • Braune, A.; Bendrat, K.; Rospert, S.; Buckel, W.
    The sodium ion translocating glutaconyl-CoA decarboxylase from Acidaminococcus fermentans: cloning and function of the genes forming a second operon (1999), Mol. Microbiol., 31, 473-487.
    View publication on PubMed
Search for more literature for 6.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
decarboxylase operon cloned and sequenced, overproduced in Escherichia coli Acidaminococcus fermentans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11624
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
14000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
14133
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
25000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
36000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
38902
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
64300
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
65000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-methylcrotonyl-CoA + HCO3- Acidaminococcus fermentans key enzyme in the fermentation of glutamate to ammonia, carbon dioxide, hydrogen, acetate and butyrate ADP + phosphate + 3-methylglutaconyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Acidaminococcus fermentans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant alpha-subunit Acidaminococcus fermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Acidaminococcus fermentans ADP + phosphate + 3-methylglutaconyl-CoA
-
 r
ATP + 3-methylcrotonyl-CoA + HCO3- key enzyme in the fermentation of glutamate to ammonia, carbon dioxide, hydrogen, acetate and butyrate Acidaminococcus fermentans ADP + phosphate + 3-methylglutaconyl-CoA
-
 r
glutaconyl-CoA + H+
-
 Acidaminococcus fermentans crotonyl-CoA + CO2
-
 r

Subunits

Subunits Comment Organism
tetramer 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans

Synonyms

Synonyms Comment Organism
glutaconyl-CoA decarboxylase
-
 Acidaminococcus fermentans
glutaconyl-CoA:biotin carboxytransferase
-
 Acidaminococcus fermentans