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Literature summary for 6.4.1.4 extracted from
- Purification and characterization of 3-methylcrotonyl-coenzyme A carboxylase from higher plant mitochondria (1993), Plant Physiol., 102, 957-965.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | ADP and phosphate inhibit enzyme activity in an additive mode | Pisum sativum |  |
| KCl | 1.2fold stimulation in presence of 20 mM KCl. Inhibition at concentrations higher than 50 mM | Pisum sativum | |
| KCl | 1.2fold stimulation in presence of 20 mM KCl. Inhibition at concentrations higher than 50 mM | Solanum tuberosum | |
| Mg2+ | above 5 mM Mg2+, at 1 mM ATP | Pisum sativum | |
| Mg2+ | above 5 mM Mg2+, at 1 mM ATP | Solanum tuberosum | |
| additional information | neither the cyclohexanedione herbicide sethoxydim nor the aryloxyphenoxypropionic acid herbicide diclofop-methyl affect enzyme activity | Pisum sativum | |
| additional information | neither the cyclohexanedione herbicide sethoxydim nor the aryloxyphenoxypropionic acid herbicide diclofop-methyl affect enzyme activity | Solanum tuberosum | |
| NEM | - |
Pisum sativum | |
| Phenylglyoxal | - |
Pisum sativum | |
| phosphate | ADP and phosphate inhibit enzyme activity in an additive mode | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
ATP | - |
Solanum tuberosum | |
| 0.1 | - |
3-methylcrotonoyl-CoA | - |
Solanum tuberosum | |
| 0.1 | - |
3-methylcrotonoyl-CoA | ATP | Pisum sativum | |
| 0.34 | - |
HCO3- | - |
Solanum tuberosum | |
| 0.9 | - |
HCO3- | - |
Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 1.1 to 1.2fold stimulation in presence of 20 mM KCl. Inhibition at concentrations higher than 50 mM | Pisum sativum | |
| K+ | 1.1 to 1.2fold stimulation in presence of 20 mM KCl. Inhibition at concentrations higher than 50 mM | Solanum tuberosum | |
| Mg2+ | maximal activation at 3 mM Mg2+ in presence of 1 mM ATP | Solanum tuberosum | |
| Mg2+ | maximal activation at 2.5 mM Mg2+ in presence of 1 mM ATP | Pisum sativum | |
| Mg2+ | Mg2+ required | Pisum sativum | |
| Mg2+ | Mg2+ required | Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
4 * 74000, biotin-containing subunit, + 4 * 53000, biotin-free subunit, SDS-PAGE | Solanum tuberosum |
| 54000 | - |
4 * 76000, biotin-containing subunit, + 4 * 54000, biotin-free subunit, SDS-PAGE | Pisum sativum |
| 74000 | - |
4 * 74000, biotin-containing subunit, + 4 * 53000, biotin-free subunit, SDS-PAGE | Solanum tuberosum |
| 76000 | - |
4 * 76000, biotin-containing subunit, + 4 * 54000, biotin-free subunit, SDS-PAGE | Pisum sativum |
| 500000 | - |
gel filtration | Solanum tuberosum |
| 530000 | - |
gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
| Solanum tuberosum | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + 3-methylcrotonoyl-CoA + HCO3- + H+ = ADP + phosphate + 3-methylglutaconyl-CoA | bi bi uni uni ping-pong mechanism, i.e. double-displacement kinetic mechanism | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.77 | - |
- |
Solanum tuberosum |
| 4.5 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pisum sativum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
| ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Solanum tuberosum | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 4 * 76000, biotin-containing subunit, + 4 * 54000, biotin-free subunit, SDS-PAGE | Pisum sativum |
| octamer | 4 * 74000, biotin-containing subunit, + 4 * 53000, biotin-free subunit, SDS-PAGE | Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pisum sativum |
| 38 | - |
- |
Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.2 | - |
Solanum tuberosum |
| 8.3 | - |
- |
Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | 1 mol of biotin per 128000 MW protein | Solanum tuberosum | |
| biotin | 1 mol of biotin per 133000 MW protein | Pisum sativum | |
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