KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
3-methylcrotonoyl-CoA | - |
Pseudomonas citronellolis | |
0.138 | - |
(E)-crotonoyl-CoA | - |
Pseudomonas citronellolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
73000 | - |
4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
520000 | 580000 | gel filtration | Pseudomonas citronellolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas citronellolis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas citronellolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.54 | - |
- |
Pseudomonas citronellolis |
Storage Stability | Organism |
---|---|
-80°C, approximately 25% loss of activity per month | Pseudomonas citronellolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Pseudomonas citronellolis | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
ATP + crotonoyl-CoA + HCO3- | (E)-crotonoyl-CoA | Pseudomonas citronellolis | ? | - |
? | |
ATP + crotonoyl-CoA + HCO3- | 44% of the activity relative to 3-methylcrotonoyl-CoA | Pseudomonas citronellolis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 4 * 63000, biotin-free subunit A, + x * 73000, biotin-containing subunit B | Pseudomonas citronellolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | enzyme contains biotin | Pseudomonas citronellolis | |
biotin | biotin is bound to the heavier subunit | Pseudomonas citronellolis |