Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2E)-3-ethylcrotonoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus | |
(2Z)-geranoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus | |
butanoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus | |
NEM | - |
Bos taurus | |
p-hydroxymercuribenzoate | preincubation of the enzyme with ATP or ADP protects the enzyme, while 3-methylcrotonoyl-CoA provides only very slight protection | Bos taurus | |
Phenylglyoxal | preincubation with ATP or ADP protects, preincubation with 3-methylcrotonoyl-CoA does not | Bos taurus | |
propanoyl-CoA | competitive with respect to 3-methylcrotonoyl-CoA | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
(2Z)-3-ethylcrotonoyl-CoA | - |
Bos taurus | |
0.075 | - |
3-methylcrotonoyl-CoA | - |
Bos taurus | |
0.082 | - |
ATP | kidney enzyme | Bos taurus | |
0.225 | - |
(E)-crotonoyl-CoA | - |
Bos taurus | |
1.8 | - |
HCO3- | kidney enzyme | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, Co2+ activates at 34% of the efficiency relative to Mg2+ | Bos taurus | |
K+ | activates 7fold at 50 mM. Relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus | |
Li+ | relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus | |
Mg2+ | divalent cation required, Mg2+ is the best activator | Bos taurus | |
Mn2+ | divalent cation required. Mn2+ activates with 85% of the efficiency relative to Mg2+ | Bos taurus | |
Na+ | relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus | |
NH4+ | relative activation by monovalent cations in decreasing order: K+/ NH4+, Na+, Li+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B | Bos taurus |
73500 | - |
x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B | Bos taurus |
835000 | - |
PAGE, gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.7 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, 4 months, less than 20% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (2Z)-3-ethylcrotonoyl-CoA | 12% of the activity relative to 3-methylcrotonoyl-CoA | Bos taurus | ADP + phosphate + 3-ethylglutaconyl-CoA | - |
? | |
ATP + 3-methylcrotonoyl-CoA + HCO3- | - |
Bos taurus | ADP + phosphate + 3-methylglutaconyl-CoA | - |
? | |
ATP + acetoacetyl-CoA + HCO3- | 12% of the activity relative to 3-methylcrotonoyl-CoA | Bos taurus | ? | - |
? | |
ATP + crotonoyl-CoA + HCO3- | (E)-crotonoyl-CoA | Bos taurus | ? | - |
? | |
ATP + crotonoyl-CoA + HCO3- | 27% of the activity relative to 3-methylcrotonoyl-CoA | Bos taurus | ? | - |
? | |
UTP + 3-methylcrotonoyl-CoA + HCO3- | at 62% of the activity relative to ATP | Bos taurus | UDP + phosphate + 3-methylglutaconyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, biotin-free subunit A, + x * 73500, biotin-containing subunit B | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Bos taurus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 50 | 23°C: 60% of maximal activity, 30°C: 75% of maximal activity, 50°C: 15% of maximal activity | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in presence of Mg2+ and K+ | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | pH 6.0: no activity, 8.0: maximal activity, pH 9.0: 50% of maximal activity | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | enzyme contains biotin | Bos taurus | |
biotin | contains 1 mol biotin per 157000 MW AB protomeric structure | Bos taurus |