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Literature summary for 6.4.1.2 extracted from
- Improved purification and further characterization of acetyl-CoA carboxylase from cultured cells of parsley (Petroselinum hortense) (1983), Eur. J. Biochem., 133, 335-339.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | stimulates at 2 mM, inhibits at higher concentrations | Petroselinum crispum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | stimulates at 2 mM, inhibits at higher concentrations | Petroselinum crispum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
ATP | - |
Petroselinum crispum | |
| 0.15 | - |
acetyl-CoA | - |
Petroselinum crispum | |
| 1 | - |
HCO3- | - |
Petroselinum crispum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Petroselinum crispum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activates | Petroselinum crispum | |
| Mg2+ | maximal activation when ATP exists largely as MgATP2- with low concentrations of free Mg2+ | Petroselinum crispum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 220000 | - |
2 * 220000, SDS-PAGE | Petroselinum crispum |
| 420000 | - |
gel filtration, sucrose density gradient sedimentation | Petroselinum crispum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petroselinum crispum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | irradiated | Petroselinum crispum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Petroselinum crispum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetyl-CoA + HCO3- | - |
Petroselinum crispum | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + acetyl-CoA + hydrogencarbonate | ATP in form of MgATP2- | Petroselinum crispum | ADP + phosphate + malonyl-CoA | - |
? | |
| ATP + butyryl-CoA + HCO3- | 15% of the activity relative to acetyl-CoA | Petroselinum crispum | ADP + ethylmalonyl-CoA + phosphate | - |
? | |
| ATP + propionyl-CoA + HCO3- | 60% of the activity relative to acetyl-CoA | Petroselinum crispum | ADP + phosphate + methylmalonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 220000, SDS-PAGE | Petroselinum crispum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Petroselinum crispum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | 25°C: about 35% of maximal activity, 45°C: maximal activity | Petroselinum crispum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Petroselinum crispum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | covalent attachment of the biotin prosthetic group | Petroselinum crispum | |
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