Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2',3'-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate | allosteric activator of pyruvate carboxylase. The increase in activity between 2 mM and saturating MgATP is approximately 16fold | Rhizobium etli | |
acetyl-CoA | allosteric activator of pyruvate carboxylase, there is a 7fold increase in the turnover number for ATP cleavage induced by acetyl-CoA | Rhizobium etli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhizobium etli |
Protein Variants | Comment | Organism |
---|---|---|
R472S | the mutation severely decreases the affinity of the enzyme for acetyl-CoA | Rhizobium etli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',3'-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate | inhibitor with respect to MgATP2- | Rhizobium etli | |
ATP | at concentrations above saturating, MgATP2- activates bicarbonate-dependent ATP cleavage, but inhibits the overall reaction | Rhizobium etli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Co2+-affinity column chromatography | Rhizobium etli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- + H+ | - |
Rhizobium etli | ADP + oxaloacetate + phosphate | - |
? | |
additional information | 2',3'-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate is no substrate | Rhizobium etli | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | at concentrations above saturating, MgATP2- activates bicarbonate-dependent ATP cleavage, but inhibits the overall reaction | Rhizobium etli |