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Literature summary for 6.4.1.1 extracted from

  • Yu, L.P.; Xiang, S.; Lasso, G.; Gil, D.; Valle, M.; Tong, L.
    A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A (2009), Structure, 17, 823-832.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.1

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with coenzyme A, symmetrical tetramer with one coenzyme A molecule bound to each monomer. Presence of acetyl-CoA promotes a conformation for the dimer of the biotin carboxylase domain of pyruvate carboxylase that might be catalytically more competent Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
A610T more than 30fold loss in catalytic efficiency Staphylococcus aureus
K912T more than 30fold loss in catalytic efficiency Staphylococcus aureus
Q870A 2fold loss in catalytic efficiency Staphylococcus aureus
R644A more than 30fold loss in catalytic efficiency Staphylococcus aureus
R644K more than 30fold loss in catalytic efficiency Staphylococcus aureus
S911A 1.5fold loss in catalytic efficiency Staphylococcus aureus
T908A more than 30fold loss in catalytic efficiency Staphylococcus aureus
Y651A more than 30fold loss in catalytic efficiency Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
 pyruvate wild-type, pH 7.5, presence of acetyl-CoA Staphylococcus aureus
1.8
-
 pyruvate mutant Q870A, pH 7.5 Staphylococcus aureus
2.3
-
 pyruvate mutant S911A, pH 7.5 Staphylococcus aureus
4.4
-
 pyruvate wild-type, pH 7.5 Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A0H3JRU9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
 Staphylococcus aureus ADP + oxaloacetate + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 pyruvate mutant Q870A, pH 7.5 Staphylococcus aureus
2.5
-
 pyruvate mutant S911A, pH 7.5 Staphylococcus aureus
6.5
-
 pyruvate wild-type, pH 7.5 Staphylococcus aureus
15.7
-
 pyruvate wild-type, pH 7.5, presence of acetyl-CoA Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 pyruvate mutant Q870A, pH 7.5 Staphylococcus aureus
1.1
-
 pyruvate mutant S911A, pH 7.5 Staphylococcus aureus
1.5
-
 pyruvate wild-type, pH 7.5 Staphylococcus aureus
27.2
-
 pyruvate wild-type, pH 7.5, presence of acetyl-CoA Staphylococcus aureus