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Literature summary for 6.4.1.1 extracted from

  • Yong-Biao, J.; Islam, M.N.; Sueda, S.; Kondo, H.
    Identification of the catalytic residues involved in the carboxyl transfer of pyruvate carboxylase (2004), Biochemistry, 43, 5912-5920.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109 Geobacillus thermodenitrificans

Protein Variants

Protein Variants Comment Organism
D543E mutant with decreased activity Geobacillus thermodenitrificans
D649N mutant with decreased activity Geobacillus thermodenitrificans
D713E mutant with decreased activity Geobacillus thermodenitrificans
D713N mutant with decreased activity Geobacillus thermodenitrificans
D762E mutant with decreased activity Geobacillus thermodenitrificans
D762N mutant with decreased activity Geobacillus thermodenitrificans
E576D mutant with decreased activity Geobacillus thermodenitrificans
E576Q mutant with decreased activity Geobacillus thermodenitrificans
E592Q mutant with decreased activity Geobacillus thermodenitrificans
K712Q mutant with decreased activity Geobacillus thermodenitrificans
K712R mutant with decreased activity Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
pyruvate D762N Geobacillus thermodenitrificans
0.29
-
pyruvate D762E Geobacillus thermodenitrificans
0.3
-
pyruvate wild type Geobacillus thermodenitrificans
0.41
-
pyruvate K712R Geobacillus thermodenitrificans
0.44
-
pyruvate E576D Geobacillus thermodenitrificans
0.49
-
pyruvate E592Q Geobacillus thermodenitrificans
0.58
-
pyruvate D713E Geobacillus thermodenitrificans
1.16
-
pyruvate D713N Geobacillus thermodenitrificans
1.2
-
pyruvate D649N Geobacillus thermodenitrificans
1.62
-
pyruvate E576Q Geobacillus thermodenitrificans
16.2
-
pyruvate D543E Geobacillus thermodenitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + HCO3- Geobacillus thermodenitrificans
-
ADP + oxaloacetate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + HCO3-
-
Geobacillus thermodenitrificans ADP + oxaloacetate + phosphate
-
?

Cofactor

Cofactor Comment Organism Structure
biotin
-
Geobacillus thermodenitrificans