Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 | Geobacillus thermodenitrificans |
Protein Variants | Comment | Organism |
---|---|---|
D543E | mutant with decreased activity | Geobacillus thermodenitrificans |
D649N | mutant with decreased activity | Geobacillus thermodenitrificans |
D713E | mutant with decreased activity | Geobacillus thermodenitrificans |
D713N | mutant with decreased activity | Geobacillus thermodenitrificans |
D762E | mutant with decreased activity | Geobacillus thermodenitrificans |
D762N | mutant with decreased activity | Geobacillus thermodenitrificans |
E576D | mutant with decreased activity | Geobacillus thermodenitrificans |
E576Q | mutant with decreased activity | Geobacillus thermodenitrificans |
E592Q | mutant with decreased activity | Geobacillus thermodenitrificans |
K712Q | mutant with decreased activity | Geobacillus thermodenitrificans |
K712R | mutant with decreased activity | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
pyruvate | D762N | Geobacillus thermodenitrificans | |
0.29 | - |
pyruvate | D762E | Geobacillus thermodenitrificans | |
0.3 | - |
pyruvate | wild type | Geobacillus thermodenitrificans | |
0.41 | - |
pyruvate | K712R | Geobacillus thermodenitrificans | |
0.44 | - |
pyruvate | E576D | Geobacillus thermodenitrificans | |
0.49 | - |
pyruvate | E592Q | Geobacillus thermodenitrificans | |
0.58 | - |
pyruvate | D713E | Geobacillus thermodenitrificans | |
1.16 | - |
pyruvate | D713N | Geobacillus thermodenitrificans | |
1.2 | - |
pyruvate | D649N | Geobacillus thermodenitrificans | |
1.62 | - |
pyruvate | E576Q | Geobacillus thermodenitrificans | |
16.2 | - |
pyruvate | D543E | Geobacillus thermodenitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | Geobacillus thermodenitrificans | - |
ADP + oxaloacetate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Geobacillus thermodenitrificans | ADP + oxaloacetate + phosphate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | - |
Geobacillus thermodenitrificans |