Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | acetyl-CoA and K+ have no effect on ADP phosphorylation by carbamoyl phosphate | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
Pyc1 isoform and C249A mutant | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C249A | only small effects on enzyme activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | pH 7.8, 30°C, C249A mutant | Saccharomyces cerevisiae | |
0.07 | - |
ATP | pH 7.8, 30°C, wild-type Pyc1 | Saccharomyces cerevisiae | |
0.45 | - |
pyruvate | pH 7.8, 30°C, C249A mutant | Saccharomyces cerevisiae | |
0.5 | - |
pyruvate | pH 7.8, 30°C, wild-type Pyc1 | Saccharomyces cerevisiae | |
1.36 | - |
HCO3- | pH 7.8, 30°C, wild-type Pyc1 | Saccharomyces cerevisiae | |
1.7 | - |
Carbamoyl phosphate | pH 7.8, 30°C, C249A mutant, with acetyl-CoA and K+ | Saccharomyces cerevisiae | |
2.3 | - |
HCO3- | pH 7.8, 30°C, C249A mutant | Saccharomyces cerevisiae | |
10.7 | - |
Carbamoyl phosphate | pH 7.8, 30°C, C249A mutant, without acetyl-CoA and K+ | Saccharomyces cerevisiae | |
11.3 | - |
Carbamoyl phosphate | pH 7.8, 30°C, wild-type Pyc1, with acetyl-CoA and K+ | Saccharomyces cerevisiae | |
12.3 | - |
Carbamoyl phosphate | pH 7.8, 30°C, wild-type Pyc1, without acetyl-CoA and K+ | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | Saccharomyces cerevisiae | - |
ADP + phosphate + oxaloacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Pyc1 isoform and C249A mutant | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + carbamoyl phosphate | - |
Saccharomyces cerevisiae | ATP + ? | - |
? | |
ATP + pyruvate + HCO3- | - |
Saccharomyces cerevisiae | ADP + phosphate + oxaloacetate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
Carbamoyl phosphate | pH 7.8, 30°C, C249A mutant, with acetyl-CoA and K+ | Saccharomyces cerevisiae | |
0.11 | - |
Carbamoyl phosphate | pH 7.8, 30°C, wild-type Pyc1, with acetyl-CoA and K+ | Saccharomyces cerevisiae | |
0.11 | - |
Carbamoyl phosphate | pH 7.8, 30°C, wild-type Pyc1, without acetyl-CoA and K+ | Saccharomyces cerevisiae | |
0.13 | - |
Carbamoyl phosphate | pH 7.8, 30°C, C249A mutant, without acetyl-CoA and K+ | Saccharomyces cerevisiae |