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Literature summary for 6.3.5.9 extracted from

  • Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.
    Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans (1990), J. Bacteriol., 172, 6239-6244.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00021
-
hydrogenobyrinic acid c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.00041
-
Hydrogenobyrinic acid pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.012
-
ammonia pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.0203
-
glutamine pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.071
-
(CN,aq)cobyrinic acid c-monoamide pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.16
-
(CN,aq)cobyrinic acid pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)
0.25
-
(aq)2cobyrinic acid pH 7.6, 30°C Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
2 * 45000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
86000
-
gel filtration Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cobyrinic acid + L-glutamine + H2O Pseudomonas denitrificans (nom. rej.) cobalamin pathway from cobyrinic acid ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O Pseudomonas denitrificans (nom. rej.)
-
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.) P21632
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (aq)2cobyrinic acid + glutamine + H2O
-
Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
?
ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
Pseudomonas denitrificans (nom. rej.) ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
?
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
?
ATP + (CN,aq)cobyrinic acid c-monoamide + L-glutamine + H2O
-
Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
?
ATP + cobyrinic acid + ammonia + H2O
-
Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
?
ATP + cobyrinic acid + L-glutamine + H2O intermediate formation of cobyrinic acid c-monoamide Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
?
ATP + cobyrinic acid + L-glutamine + H2O cobalamin pathway from cobyrinic acid Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
Pseudomonas denitrificans (nom. rej.) ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
?
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
Pseudomonas denitrificans (nom. rej.) ADP + phosphate + ?
-
?
additional information cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates Pseudomonas denitrificans (nom. rej.) ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

Synonyms Comment Organism
cobyrinic acid a,c-diamide synthase
-
Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
broad optimum with maximum activity from pH 6.8-8.0 Pseudomonas denitrificans (nom. rej.)