Cloned (Comment) | Organism |
---|---|
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation | Pseudomonas denitrificans (nom. rej.) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
hydrogenobyrinic acid c-monoamide | pH 7.6, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.00041 | - |
Hydrogenobyrinic acid | pH 7.6, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.012 | - |
ammonia | pH 7.6, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.0203 | - |
glutamine | pH 7.6, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.071 | - |
(CN,aq)cobyrinic acid c-monoamide | pH 7.6, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.16 | - |
(CN,aq)cobyrinic acid | pH 7.6, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.25 | - |
(aq)2cobyrinic acid | pH 7.6, 30°C | Pseudomonas denitrificans (nom. rej.) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas denitrificans (nom. rej.) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
86000 | - |
gel filtration | Pseudomonas denitrificans (nom. rej.) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cobyrinic acid + L-glutamine + H2O | Pseudomonas denitrificans (nom. rej.) | cobalamin pathway from cobyrinic acid | ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate | - |
? | |
ATP + hydrogenobyrinic acid + L-glutamine + H2O | Pseudomonas denitrificans (nom. rej.) | - |
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | P21632 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (aq)2cobyrinic acid + glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + ? | - |
? | |
ATP + (CN,aq)cobyrinic acid + glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ? | - |
? | |
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + ? | - |
? | |
ATP + (CN,aq)cobyrinic acid c-monoamide + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + ? | - |
? | |
ATP + cobyrinic acid + ammonia + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + ? | - |
? | |
ATP + cobyrinic acid + L-glutamine + H2O | intermediate formation of cobyrinic acid c-monoamide | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate | - |
? | |
ATP + cobyrinic acid + L-glutamine + H2O | cobalamin pathway from cobyrinic acid | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate | - |
? | |
ATP + hydrogenobyrinic acid + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate | - |
? | |
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + ? | - |
? | |
additional information | cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates | Pseudomonas denitrificans (nom. rej.) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Synonyms | Comment | Organism |
---|---|---|
cobyrinic acid a,c-diamide synthase | - |
Pseudomonas denitrificans (nom. rej.) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
broad optimum with maximum activity from pH 6.8-8.0 | Pseudomonas denitrificans (nom. rej.) |