Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.5.6 extracted from

  • Bailly, M.; Blaise, M.; Lorber, B.; Becker, H.D.; Kern, D.
    The transamidosome: a dynamic ribonucleoprotein particle dedicated to prokaryotic tRNA-dependent asparagine biosynthesis (2007), Mol. Cell, 28, 228-239.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state und pre-steady-state kinetics, association of dimeric DRS2, AdT, and tRNAAsn in a ternary complex, AdT binds tRNAAsn with a lower affinity than DRS2, overview Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Asp-tRNA + L-Gln in presence of tRNA-dependent amidotransferase AdT, amidation activity Thermus thermophilus ?
-
?
additional information a nondiscriminating aspartyl-tRNA synthetase, ND-DRS, first generates a mischarged aspartyl-tRNAAsn that dissociates from the enzyme and binds to a tRNA-dependent amidotransferase, AdT, which then converts the tRNA-bound aspartate into asparagine, the ND-DRS, tRNAAsn, and AdT assemble into a specific ribonucleoprotein complex called transamidosome that remains stable during the overall catalytic process, overview Thermus thermophilus ?
-
?

Subunits

Subunits Comment Organism
More association of dimeric DRS2, AdT, and tRNAAsn in a ternary complex: a nondiscriminating aspartyl-tRNA synthetase, ND-DRS, first generates a mischarged aspartyl-tRNAAsn that dissociates from the enzyme and binds to a tRNA-dependent amidotransferase, AdT, which then converts the tRNA-bound aspartate into asparagine, the ND-DRS, tRNAAsn, and AdT assemble into a specific ribonucleoprotein complex called transamidosome that remains stable during the overall catalytic process, overview Thermus thermophilus

Synonyms

Synonyms Comment Organism
tRNA-dependent amidotransferase
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermus thermophilus