Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3), co-expression with the cytotoxic nondiscriminating aspartyl-tRNA synthetase, EC 6.1.1.23, which is toxic for the cells, rescues the toxicity, overview | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartyl-tRNAAsn + L-glutamine | Helicobacter pylori | - |
ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartyl-tRNAAsn + L-glutamine | - |
Helicobacter pylori | ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate | - |
? | |
ATP + L-aspartyl-tRNAAsn + L-glutamine | the Halobacter pylori Asp/Glu-Adt can utilize Escherichia coli Asp-tRNAAsn as a substrate, recombinantly produced tRNA substrate | Helicobacter pylori | ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate | - |
? | |
additional information | in bacteria that lack AsnRS, EC 6.1.1.12, AspRS is nondiscriminating, EC 6.1.1.23, and generates both Asp-tRNAAsp and the noncanonical, misacylated Asp-tRNAAsn, this misacylated tRNA is subsequently repaired by the glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, overview | Helicobacter pylori | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asp/Glu-Adt | - |
Helicobacter pylori |
glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase | - |
Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Helicobacter pylori |