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Literature summary for 6.3.5.6 extracted from

  • Tumbula-Hansen, D.; Feng, L.; Toogood, H.; Stetter, K.O.; Söll, D.
    Evolutionary divergence of the archaeal aspartyl-tRNA synthetases into discriminating and nondiscriminating forms (2002), J. Biol. Chem., 277, 37184-37190.
    View publication on PubMed
Search for more literature for 6.3.5.6

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the gatCAB operon encoding the Asp-tRNAAsn amidotransferase and expression in Escherichia coli BL21-Codon Plus(DE3)-RIL Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Asp-tRNAAsn + ? Methanothermobacter thermautotrophicus the mischarging Asp-tRNA synthetase correlates with the absence in the genome of Asn-tRNA synthetase and the presence of Asp-tRNAAsn amidotransferase, employed by the transamidation pathway Asn-tRNAAsn + ?
-
 ?
Asp-tRNAAsn + ? Methanothermobacter thermautotrophicus enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Asn-tRNAAsn + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme, expressed in Escherichia coli Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asp-tRNAAsn + ? the mischarging Asp-tRNA synthetase correlates with the absence in the genome of Asn-tRNA synthetase and the presence of Asp-tRNAAsn amidotransferase, employed by the transamidation pathway Methanothermobacter thermautotrophicus Asn-tRNAAsn + ?
-
 ?
Asp-tRNAAsn + ? enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Methanothermobacter thermautotrophicus Asn-tRNAAsn + ?
-
 ?
ATP + Asp-tRNAAsn + glutamine
-
 Methanothermobacter thermautotrophicus ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?

Subunits

Subunits Comment Organism
heterotrimer GatCAB enzyme Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at, as a compromise between optimal enzyme activity and the stability of the reaction product Methanothermobacter thermautotrophicus