Cloned (Comment) | Organism |
---|---|
gatCAB genes encoding Glu/Asp-AdT are cloned and expressed in Escherichia coli | Deinococcus radiodurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Asp-tRNAAsn + ? | Deinococcus radiodurans | gatCAB genes encoded Asp-AdT may be responsible for biosynthesis of asparagine, glutamine as amide source | Asn-tRNAAsn + ? | - |
? | |
Asp-tRNAAsn + ? | Deinococcus radiodurans | enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase | Asn-tRNAAsn + ? | - |
? | |
Asp-tRNAAsn + ? | Deinococcus radiodurans | in vivo only Asn-tRNAAsn formation, because the substrate availability of Asp-tRNAAsn allows only one final product | Asn-tRNAAsn + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Deinococcus radiodurans | - |
- |
- |
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | - |
- |
- |
Haloferax volcanii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of recombinant enzyme, expressed in Escherichia coli | Deinococcus radiodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Asp-tRNAAsn + ? | gatCAB genes encoded Asp-AdT may be responsible for biosynthesis of asparagine, glutamine as amide source | Deinococcus radiodurans | Asn-tRNAAsn + ? | - |
? | |
Asp-tRNAAsn + ? | enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase | Deinococcus radiodurans | Asn-tRNAAsn + ? | - |
? | |
Asp-tRNAAsn + ? | in vivo only Asn-tRNAAsn formation, because the substrate availability of Asp-tRNAAsn allows only one final product | Deinococcus radiodurans | Asn-tRNAAsn + ? | - |
? | |
ATP + Asp-tRNAAsn + glutamine | - |
Bacillus subtilis | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
ATP + Asp-tRNAAsn + glutamine | - |
Haloferax volcanii | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
ATP + Asp-tRNAAsn + glutamine | single enzyme with both Gln-tRNA and Asp-tRNA transamidation activities, but functions as Asp-AdT, since Deinococcus do not produce the required mischarged Glu-tRNAGln substrate | Deinococcus radiodurans | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
ATP + Asp-tRNAAsn + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Deinococcus radiodurans | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
ATP + Asp-tRNAAsn + glutamine | single enzyme with both Gln-tRNA and Asp-tRNA transamidation activities, but functions as Asp-AdT, since Deinococcus do not produce the required mischarged Glu-tRNAGln substrate | Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
ATP + Asp-tRNAAsn + glutamine | dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA | Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? | |
ATP + Glu-tRNAGln + glutamine | dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT | Deinococcus radiodurans | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
ATP + Glu-tRNAGln + glutamine | in vitro both Asp-tRNAAsn and Glu-tRNAGln amidation activities, in vivo only Gln-tRNAGln formation | Bacillus subtilis | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
ATP + Glu-tRNAGln + glutamine | Asp/Glu-AdT carries out Gln-tRNA formation, since Bacillus contains a non-discriminating GluRS, but lacks a mischarging AspRS | Bacillus subtilis | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
ATP + Glu-tRNAGln + glutamine | dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT | Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ADP + Gln-tRNAGln + glutamate + phosphate | - |
? | |
additional information | A subunit is a tRNA-independent glutaminase, B subunit is a putative tRNA-binding protein | Deinococcus radiodurans | ? | - |
? | |
additional information | A subunit is a tRNA-independent glutaminase, B subunit is a putative tRNA-binding protein | Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Deinococcus radiodurans |