Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IMP | the IMP activator and the UMP inhibitor bind to the same site on the enzyme | Escherichia coli | |
ornithine | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Echerichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G1008A | mutation abolishs IMP activation and UMP inhibition in comparison to wild-type enzyme | Escherichia coli |
G997A | mutation abolishs IMP activation and UMP inhibition in comparison to wild-type enzyme | Escherichia coli |
H995A | mutation has little effect on enzyme activity in comparison to wild-type enzyme | Escherichia coli |
K1061A | mutation abolishs IMP activation and UMP inhibition in comparison to wild-type enzyme | Escherichia coli |
K954A | mutation has little effect on enzyme activity in comparison to wild-type enzyme | Escherichia coli |
K993A | mutation reduces enzyme activity in comparison to wild-type enzyme | Escherichia coli |
K993W | mutation has little effect on enzyme activity in comparison to wild-type enzyme | Escherichia coli |
K993W/H995A | mutation abolishs IMP activation and UMP inhibition in comparison to wild-type enzyme | Escherichia coli |
L990A | mutation abolishs IMP activation and UMP inhibition in comparison to wild-type enzyme | Escherichia coli |
R1020A | mutation has little effect on enzyme activity in comparison to wild-type enzyme | Escherichia coli |
R1021A | mutation has little effect on enzyme activity in comparison to wild-type enzyme | Escherichia coli |
S948A | mutation has little effect on enzyme activity in comparison to wild-type enzyme | Escherichia coli |
T974A | mutation abolishs IMP activation and UMP inhibition in comparison to wild-type enzyme | Escherichia coli |
V991A | mutation has little effect on enzyme activity in comparison to wild-type enzyme | Escherichia coli |
V994A | mutation reduces enzyme activity in comparison to wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UMP | the IMP activator and the UMP inhibitor bind to the same site on the enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41400 | - |
1 * 41400 + 1 * 117700, the large subunit catalyses the carbamoyl phosphate synthesis from ammonia in three steps, and binds the effectors in its 15000 domain | Escherichia coli |
117700 | - |
1 * 41400 + 1 * 117700, the large subunit catalyses the carbamoyl phosphate synthesis from ammonia in three steps, and binds the effectors in its 15000 domain | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | Escherichia coli | enzyme is a key enzyme in the pyrimidine nucleotide and arginine biosynthetic pathways | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | Escherichia coli L814 | enzyme is a key enzyme in the pyrimidine nucleotide and arginine biosynthetic pathways | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
L814 | - |
Escherichia coli L814 | - |
L814 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | enzyme is a key enzyme in the pyrimidine nucleotide and arginine biosynthetic pathways | Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | - |
Escherichia coli L814 | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | enzyme is a key enzyme in the pyrimidine nucleotide and arginine biosynthetic pathways | Escherichia coli L814 | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli L814 | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 41400 + 1 * 117700, the large subunit catalyses the carbamoyl phosphate synthesis from ammonia in three steps, and binds the effectors in its 15000 domain | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
UMP | pH 8.0, 37°C, wild-type enzyme in comparison to mutant enzymes | Escherichia coli |