Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IMP | the effector stabilzes the (alpha,beta)4-tetramer of enzyme | Escherichia coli | |
L-ornithine | the effector stabilizes the (alpha,beta)4-tetramer of enzyme | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L421E | mutant forms a (alpha/beta)2-dimer in presence of ornithine, IMP and UMP | Escherichia coli |
L421E/N987D | no oligomerization | Escherichia coli |
N987D | mutant forms a (alpha/beta)-monomer regardless of the presence of any allosteric effectors | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UMP | the inhibitor stabilizes the (alpha,beta)2-dimer of enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
ATP | pH 7.6, wild-type enzyme in presence of ornithine and in comparison to mutant enzymes | Escherichia coli | |
0.37 | - |
ATP | pH 7.6, wild-type enzyme in presence of IMP and in comparison to mutant enzymes | Escherichia coli | |
0.47 | - |
ATP | pH 7.6, wild-type enzyme in absence of effectors and in comparison to mutant enzymes | Escherichia coli | |
1.6 | - |
ATP | pH 7.6, wild-type enzyme in presence of UMP and in comparison to mutant enzymes | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
alpha,beta, 1 * 42000 + 1 * 118000, the effectors ornithine, IMP and UMP modulate the oligomerization of the heterodimer to higher ordered species | Escherichia coli |
118000 | - |
alpha,beta, 1 * 42000 + 1 * 118000, the effectors ornithine, IMP and UMP modulate the oligomerization of the heterodimer to higher ordered species | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | Escherichia coli | - |
2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the catalytic activity is dependent on the presence or absence of specific allosteric ligands but independent of the oligomeric state of the protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | alpha,beta, 1 * 42000 + 1 * 118000, the effectors ornithine, IMP and UMP modulate the oligomerization of the heterodimer to higher ordered species | Escherichia coli |