Protein Variants | Comment | Organism |
---|---|---|
C269S | mutant is unable to hydrolyse glutamine, the mutant is proved to be an adequate mimic for studies of the Michaelis complex | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | Escherichia coli | the product carbamoyl phosphate is an essential precursor of arginine and pyrimidine biosynthesis | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate | steps of reaction pathway for glutamine hydrolysis, the Michalis complex, the tetrahedral intermediate and glutamyl-thioester intermediate models, stereochemistry of the overall glutaminase reaction | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + L-Gln + HCO3- | the product carbamoyl phosphate is an essential precursor of arginine and pyrimidine biosynthesis | Escherichia coli | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? |