Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.5.5 extracted from

  • Yang, H.; Park, S.M.; Nolan, W.G.; Lu, C.D.; Abdelal, A.T.
    Cloning and characterization of the arginine-specific carbamoyl-phosphate synthetase from Bacillus stearothermophilus (1997), Eur. J. Biochem., 249, 443-449.
    View publication on PubMed
Search for more literature for 6.3.5.5

Activating Compound

Activating Compound Comment Organism Structure
5-phospho-alpha-D-ribosyl 1-diphosphate pyrimidine-specific carbamoyl-phosphate synthase is activated, arginine-specific enzyme not Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
carbamoyl-phosphate synthase belonging to the pyrimidine pathway Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
Arg arginine-specific carbamoyl-phosphate synthase is repressed, pyrimidine-specific enzyme not Geobacillus stearothermophilus
UMP carbamoyl-phosphate synthase belonging to the pyrimidine pathway, carbamoyl-phosphate synthase belonging to the arginine pathway is not inhibited Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 L-Gln pyrimidine-specific carbamoyl-phosphate synthase Geobacillus stearothermophilus
0.6
-
 L-Gln arginine specific carbamoyl-phosphate synthase Geobacillus stearothermophilus
9.8
-
 NH4+ carbamoyl-phosphate synthase belonging to the pyrimidine pathway Geobacillus stearothermophilus
22
-
 L-Gln carbamoyl-phosphate synthase belonging to the arginine pathway Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
 000 + 1 * 118000, carbamoyl-phosphate synthase belonging to the pyrimidine pathway, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 2 carbamoyl-phosphate synthases, one specific for pyrimidine biosynthesis and the other for arginine biosynthesis
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
 Geobacillus stearothermophilus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
2 ATP + NH4+ + HCO3-
-
 Geobacillus stearothermophilus 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 000 + 1 * 118000, carbamoyl-phosphate synthase belonging to the pyrimidine pathway, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the thermal stability of cloned enzyme is identical regardless of the growth temperature of Bacillus stearothermophilus between 42°C and 63°C. The thermal stability of the cloned enzyme is not affected by expression at 37°C in Bacillus subtilis or E. coli Geobacillus stearothermophilus