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Literature summary for 6.3.5.4 extracted from

  • Li, Z.; Zhu, L.; Xu, J.; Mon, H.; Lee, J.M.; Kusakabe, T.
    Amino Acid deprivation-induced expression of asparagine synthetase regulates the growth and survival of cultured silkworm cells (2013), Arch. Insect Biochem. Physiol., 83, 57-68.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
from BmN4 cells, recombinant expression of the GFP-tagged or Venus fluorescent protein-tagged enzyme in BmN4 cells Bombyx mori

Protein Variants

Protein Variants Comment Organism
additional information RNA interference is used to silence BmASNS expression in silkworm cells Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62800
-
x * 62800, about, sequence calculation Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + L-glutamine + H2O Bombyx mori
-
AMP + diphosphate + L-asparagine + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Bombyx mori Q2L9S5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
BmN4 cell
-
Bombyx mori
-
BmN4-SID1 cell the cell line expresses a Caenorhabditis elegans SID-1 (CeSID-1) transmembrane protein with the ability to uptake double-stranded RNA into the cells Bombyx mori
-
cell culture
-
Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + L-glutamine + H2O
-
Bombyx mori AMP + diphosphate + L-asparagine + L-glutamate
-
?

Subunits

Subunits Comment Organism
? x * 62800, about, sequence calculation Bombyx mori

Synonyms

Synonyms Comment Organism
ASNS
-
Bombyx mori
Asparagine synthetase
-
Bombyx mori

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bombyx mori

General Information

General Information Comment Organism
malfunction silencing of the BmASNS gene enhances the sensitivity of silkworm cells to amino acid starvation. Ectopic overexpression of BmASNS gene effectively inhibits cell growth in silkworm cells, whereas its overexpression can rescue cell growth upon amino acid deprivation treatment. Silkworm cells lacking BmASNS under the condition of amino acid deprivation show severely impaired proliferation Bombyx mori
physiological function BmAsns protein negatively regulates silkworm cell proliferation. The recovery of cell growth by overexpressed BmAsns protein is due to the rapid turnover of autophagic vacuoles in the cells Bombyx mori