Cloned (Comment) | Organism |
---|---|
from BmN4 cells, recombinant expression of the GFP-tagged or Venus fluorescent protein-tagged enzyme in BmN4 cells | Bombyx mori |
Protein Variants | Comment | Organism |
---|---|---|
additional information | RNA interference is used to silence BmASNS expression in silkworm cells | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62800 | - |
x * 62800, about, sequence calculation | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine + H2O | Bombyx mori | - |
AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | Q2L9S5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BmN4 cell | - |
Bombyx mori | - |
BmN4-SID1 cell | the cell line expresses a Caenorhabditis elegans SID-1 (CeSID-1) transmembrane protein with the ability to uptake double-stranded RNA into the cells | Bombyx mori | - |
cell culture | - |
Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine + H2O | - |
Bombyx mori | AMP + diphosphate + L-asparagine + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62800, about, sequence calculation | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
ASNS | - |
Bombyx mori |
Asparagine synthetase | - |
Bombyx mori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bombyx mori |
General Information | Comment | Organism |
---|---|---|
malfunction | silencing of the BmASNS gene enhances the sensitivity of silkworm cells to amino acid starvation. Ectopic overexpression of BmASNS gene effectively inhibits cell growth in silkworm cells, whereas its overexpression can rescue cell growth upon amino acid deprivation treatment. Silkworm cells lacking BmASNS under the condition of amino acid deprivation show severely impaired proliferation | Bombyx mori |
physiological function | BmAsns protein negatively regulates silkworm cell proliferation. The recovery of cell growth by overexpressed BmAsns protein is due to the rapid turnover of autophagic vacuoles in the cells | Bombyx mori |