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Literature summary for 6.3.5.4 extracted from

  • Richards, N.G.J.; Schuster, S.M.
    Mechanistic issues in asparagine synthetase catalysis (1998), Adv. Enzymol. Relat. Areas Mol. Biol., 72, 145-198.
    View publication on PubMed
Search for more literature for 6.3.5.4

Protein Variants

Protein Variants Comment Organism
E317A diminished glutaminase activity Escherichia coli
R322Q 60000fold decrease in kcat/KM for ATP-diphsphate exchange Escherichia coli
R325A no asparagine synthetase activity, glutaminase activity is retained Escherichia coli
R325K no asparagine synthetase activity, glutaminase activity is retained Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
beta-methylaspartate weak inhibitor Escherichia coli
pyrrolidine-2,3-dicarboxylic acid weak inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 ATP glutamine dependent asparagine synthetase activity Escherichia coli
0.68
-
 L-aspartate glutamine dependent asparagine synthetase activity Escherichia coli
0.69
-
 L-glutamine glutamine dependent asparagine synthetase activity Escherichia coli
1.39
-
 L-glutamine glutaminase activity in the presence of ATP Escherichia coli
1.9
-
 L-glutamine glutaminase activity in the absence of ATP Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + L-glutamine Escherichia coli
-
 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
ATP + L-aspartate + NH3 Escherichia coli asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP AMP + diphosphate + L-asparagine
-
 ?
L-glutamine Escherichia coli
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + L-glutamine
-
 Escherichia coli AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
ATP + L-aspartate + NH3 asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP Escherichia coli AMP + diphosphate + L-asparagine
-
 ?
L-glutamine
-
 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
AS-B
-
 Escherichia coli
Asparagine synthetase B
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 L-glutamine glutaminase activity in the absence of ATP Escherichia coli
1.01
-
 L-glutamine glutamine dependent asparagine synthetase activity Escherichia coli
1.05
-
 L-aspartate glutamine dependent asparagine synthetase activity Escherichia coli
1.1
-
 ATP glutamine dependent asparagine synthetase activity Escherichia coli
1.38
-
 L-glutamine glutaminase activity in the presence of ATP Escherichia coli
6.08
-
 L-glutamine glutaminase activity in the presence of ATP Escherichia coli
6.08
-
 L-glutamine glutamine dependent asparagine synthetase activity Escherichia coli