Literature summary for 6.3.5.4 extracted from

Sheng, S.; Moraga-Amador, D.A.; van Heeke, G.; Allison, R.D.; Richards, N.G.J.; Schuster, S.M.
Glutamine inhibits the ammonia-dependent activities of two Cys-1 mutants of human asparagine synthetase through the formation of an abortive complex (1993), J. Biol. Chem., 268, 16771-16780.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of several mutant enymes in Saccharomyces cerevisiae	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C1A	altering Cys-1 to either Ala or Ser eliminated the Gln-dependent activity, while only minimally affecting the kinetic properties of the NH4+-dependent reaction	Homo sapiens
C1S	altering Cys-1 to either Ala or Ser eliminated the Gln-dependent activity, while only minimally affecting the kinetic properties of the NH4+-dependent reaction	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Gln	0.4-2.0 mM, inhibits the ammonia-dependent production of Asn	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-Asp + L-Gln	-	Homo sapiens	AMP + diphosphate + Asn + Glu	-	?
ATP + L-Asp + NH3	-	Homo sapiens	AMP + diphosphate + Asn	-	?

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Release 2023.1 (January 2023)