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Literature summary for 6.3.5.2 extracted from

  • Welin, M.; Lehtiö, L.; Johansson, A.; Flodin, S.; Nyman, T.; Trésaugues, L.; Hammarström, M.; Gräslund, S.; Nordlund, P.
    Substrate specificity and oligomerization of human GMP synthetase (2013), J. Mol. Biol., 425, 4323-4333.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) gold pRARE cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with XMP, sitting drop vapor diffusion method, using 0.1 M sodium acetate trihydrate, pH 5.2, 1.6 M ammonium sulfate, and 0.2 M sodium chloride, at 25Ā°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
2 * 70000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + XMP + L-glutamine + H2O Homo sapiens the N-terminal glutaminase domain of the enzyme generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate to form GMP AMP + diphosphate + GMP + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P49915
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-charged HiTrap chelating column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + XMP + L-glutamine + H2O the N-terminal glutaminase domain of the enzyme generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate to form GMP Homo sapiens AMP + diphosphate + GMP + L-glutamate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 70000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GMP synthetase
-
Homo sapiens
GMPS
-
Homo sapiens
Guanine monophosphate synthetase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens