Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) gold pRARE cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with XMP, sitting drop vapor diffusion method, using 0.1 M sodium acetate trihydrate, pH 5.2, 1.6 M ammonium sulfate, and 0.2 M sodium chloride, at 25Ā°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + XMP + L-glutamine + H2O | Homo sapiens | the N-terminal glutaminase domain of the enzyme generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate to form GMP | AMP + diphosphate + GMP + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49915 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-charged HiTrap chelating column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + XMP + L-glutamine + H2O | the N-terminal glutaminase domain of the enzyme generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate to form GMP | Homo sapiens | AMP + diphosphate + GMP + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 70000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GMP synthetase | - |
Homo sapiens |
GMPS | - |
Homo sapiens |
Guanine monophosphate synthetase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |