Cloned (Comment) | Organism |
---|---|
cobQ gene | Pseudomonas denitrificans (nom. rej.) |
General Stability | Organism |
---|---|
20% w/v and 1 mM dithiothreitol are necessary to stabilize enzyme during purification | Pseudomonas denitrificans (nom. rej.) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas denitrificans (nom. rej.) | |
0.00104 | - |
adenosylcobyrinic acid a,c-diamide | pH 7.5 | Pseudomonas denitrificans (nom. rej.) | |
0.00121 | - |
adenosylcobyrinic acid tetraamide | pH 7.5 | Pseudomonas denitrificans (nom. rej.) | |
0.00122 | - |
adenosylcobyrinic acid triamide | pH 7.5 | Pseudomonas denitrificans (nom. rej.) | |
0.00125 | - |
adenosylcobyrinic acid pentaamide | pH 7.5 | Pseudomonas denitrificans (nom. rej.) | |
0.011 | - |
ATP | pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
0.033 | - |
L-glutamine | pH 7.5, adenosylcobyrinic acid triamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
0.035 | - |
ATP | pH 7.5, adenosylcobyrinic acid triamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
0.045 | - |
L-glutamine | pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
0.049 | - |
L-glutamine | pH 7.5, adenosylcobyrinic acid pentaamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
0.064 | - |
L-glutamine | pH 7.5, adenosylcobyrinic acid tetraamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
0.088 | - |
ATP | pH 7.5, adenosylcobyrinic acid pentaamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
0.12 | - |
ATP | pH 7.5, adenosylcobyrinic acid tetraamide as substrate | Pseudomonas denitrificans (nom. rej.) | |
20.4 | - |
ammonia | pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate | Pseudomonas denitrificans (nom. rej.) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+-dependent | Pseudomonas denitrificans (nom. rej.) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
predicted from DNA sequence of the cobQ gene | Pseudomonas denitrificans (nom. rej.) |
57000 | - |
2 * 57000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
97300 | - |
gel filtration | Pseudomonas denitrificans (nom. rej.) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O | Pseudomonas denitrificans (nom. rej.) | biosynthesis of vitamin B12, cobalamin biosynthetic pathway | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O | Pseudomonas denitrificans (nom. rej.) SC510 RifT | biosynthesis of vitamin B12, cobalamin biosynthetic pathway | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
Pseudomonas denitrificans (nom. rej.) | - |
recombinant strain SC510 Rifr(pXL617) | - |
Oxidation Stability | Organism |
---|---|
sensitive to oxidation | Pseudomonas denitrificans (nom. rej.) |
Purification (Comment) | Organism |
---|---|
45fold | Pseudomonas denitrificans (nom. rej.) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.083 | - |
pH 7.5, adenosylcobyrinic acid a,c-diamide as substrate | Pseudomonas denitrificans (nom. rej.) |
Storage Stability | Organism |
---|---|
-20°C or 0°C, purified enzyme, 0.1 M Tris hydrochloride, pH 7.5, 48 h, 100% loss of activity | Pseudomonas denitrificans (nom. rej.) |
-20°C, purified enzyme, 50 mM Tris hydrochloride, pH 7.5, 0.1 M sodium chloride, 1 mM dithiothreitol, 20% glycerol, several weeks, stable | Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | adenosylcobyric acid + ADP + phosphate + L-glutamate | - |
? | |
adenosylcobyrinic acid a,c-diamide + ATP + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) SC510 RifT | adenosylcobyric acid + ADP + phosphate + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid a,c-diamide + ammonia + H2O | ammonia can replace L-glutamine as amide group donor, but L-glutamine is preferred | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + adenosylcobyric acid + ? | - |
? | |
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O | biosynthesis of vitamin B12, cobalamin biosynthetic pathway | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O | enzyme catalyzes the stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide, four-step amidation sequence from cobyrinic acid a,c-diamide to cobyric acid via the formation of corbyrinic acid triamide, tetraamide, and pentaamide intermediates, the amidations are carried out in a specific order, enzyme is specific to coenzyme forms of substrates, no amidation of the carboxyl group at position f, L-glutamine is the preferred amide group donor | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O | biosynthesis of vitamin B12, cobalamin biosynthetic pathway | Pseudomonas denitrificans (nom. rej.) SC510 RifT | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid a,c-diamide + L-glutamine + H2O | enzyme catalyzes the stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide, four-step amidation sequence from cobyrinic acid a,c-diamide to cobyric acid via the formation of corbyrinic acid triamide, tetraamide, and pentaamide intermediates, the amidations are carried out in a specific order, enzyme is specific to coenzyme forms of substrates, no amidation of the carboxyl group at position f, L-glutamine is the preferred amide group donor | Pseudomonas denitrificans (nom. rej.) SC510 RifT | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid pentaamide + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid tetraamide + L-glutamine + H2O | two-step amidation | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
ATP + adenosylcobyrinic acid triamide + L-glutamine + H2O | three-step amidation | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + adenosylcobyric acid + L-glutamate | - |
? | |
cobinic acid a,c-diamide + ATP + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | cobinamide + ADP + phosphate + L-glutamate | - |
? | |
cobinic acid a,c-diamide + ATP + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) SC510 RifT | cobinamide + ADP + phosphate + L-glutamate | - |
? | |
additional information | no amidation of adenosylcobyric acid, hydrogenobyrinic acid a,c-diamide, monocyano or diaqua form of cobyrinic acid a,c-diamide | Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
additional information | no amidation of adenosylcobyric acid, hydrogenobyrinic acid a,c-diamide, monocyano or diaqua form of cobyrinic acid a,c-diamide | Pseudomonas denitrificans (nom. rej.) SC510 RifT | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
homodimer | 2 * 57000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Synonyms | Comment | Organism |
---|---|---|
CobQ | - |
Pseudomonas denitrificans (nom. rej.) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
broad pH-optimum around | Pseudomonas denitrificans (nom. rej.) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP-dependent | Pseudomonas denitrificans (nom. rej.) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | isoelectric focusing | - |
6 |