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Literature summary for 6.3.4.5 extracted from

  • Van de Casteele, M.; Demarez, M.; Legrain, C.; Glansdorff, N.; Pierard, A.
    Pathways of arginine biosynthesis in extreme thermophilic archaeo- and eubacteria (1990), J. Gen. Microbiol., 136, 1177-1183.
No PubMed abstract available
Search for more literature for 6.3.4.5

Inhibitors

Inhibitors Comment Organism Structure
L-arginine
-
 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-citrulline + L-aspartate Pyrococcus furiosus
-
 AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-aspartate Saccharolobus solfataricus Sulfolobus solfataricus lacks ornithine acetyltransferase and thus forms N-acetylglutamate exclusively via the energetically less favourable reaction catalysed by N-acetylglutamate synthase, investing 1 mol of acetyl CoA per mol of N-acetyl intermediate synthesized AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-aspartate Saccharolobus solfataricus P1 Sulfolobus solfataricus lacks ornithine acetyltransferase and thus forms N-acetylglutamate exclusively via the energetically less favourable reaction catalysed by N-acetylglutamate synthase, investing 1 mol of acetyl CoA per mol of N-acetyl intermediate synthesized AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
 pH 8.7, 70°C, activity in cell culture in growth medium 0.5% peptone and 0.1% yeast extract Pyrococcus furiosus
0.00045
-
 pH 8.7, 70°C, activity in cell culture in growth medium with 0.2% yeast extract Saccharolobus solfataricus
0.0007
-
 pH 8.7, 70°C, activity in cell culture in minimal growth medium with 20 mM glucose and 5 mM NH4+ Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-citrulline + L-aspartate
-
 Pyrococcus furiosus AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-aspartate
-
 Saccharolobus solfataricus AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-aspartate Sulfolobus solfataricus lacks ornithine acetyltransferase and thus forms N-acetylglutamate exclusively via the energetically less favourable reaction catalysed by N-acetylglutamate synthase, investing 1 mol of acetyl CoA per mol of N-acetyl intermediate synthesized Saccharolobus solfataricus AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-aspartate
-
 Saccharolobus solfataricus P1 AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
ATP + L-citrulline + L-aspartate Sulfolobus solfataricus lacks ornithine acetyltransferase and thus forms N-acetylglutamate exclusively via the energetically less favourable reaction catalysed by N-acetylglutamate synthase, investing 1 mol of acetyl CoA per mol of N-acetyl intermediate synthesized Saccharolobus solfataricus P1 AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?

Synonyms

Synonyms Comment Organism
Argininosuccinate synthetase
-
 Pyrococcus furiosus
Argininosuccinate synthetase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Pyrococcus furiosus
70
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 assay at Pyrococcus furiosus
8.7
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
 L-arginine pH 8.7, 70°C Saccharolobus solfataricus

Expression

Organism Comment Expression
Saccharolobus solfataricus threefold repression of enzyme formation by arginine additional information

General Information

General Information Comment Organism
metabolism Sulfolobus solfataricus lacks ornithine acetyltransferase and thus forms N-acetylglutamate exclusively via the energetically less favourable reaction catalysed by N-acetylglutamate synthase, investing 1 mol of acetyl CoA per mol of N-acetyl intermediate synthesized Saccharolobus solfataricus