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Literature summary for 6.3.4.5 extracted from
- Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production (2009), J. Biol. Chem., 284, 20022-20033.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | interaction with Bj-BPP-10c, snake venom anti-hypertensive peptide of Botrops jararaca, activates the catalytic activity of argininosuccinate snythetase in a dose-dependent manner | Homo sapiens | |
| additional information | interaction with Bj-BPP-10c, snake venom anti-hypertensive peptide of Botrops jararaca, activates the catalytic activity of argininosuccinate snythetase in a dose-dependent manner | Mus musculus |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | novel target for the therapy of hypertension-related disorders | Homo sapiens |
| medicine | novel target for the therapy of hypertension-related disorders | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
aspartate | in presence of 0.0005 mM Bj-BPP-10c | Mus musculus |  |
| 0.15 | - |
ATP | in presence of 0.0005 mM Bj-BPP-10c | Mus musculus | |
| 0.17 | - |
aspartate | in presence of 0.1 mM Bj-BPP-10c | Mus musculus | |
| 0.18 | - |
aspartate | - |
Mus musculus | |
| 0.18 | - |
citrulline | in presence of 0.0005 mM Bj-BPP-10c | Mus musculus | |
| 0.29 | - |
citrulline | in presence of 0.1 mM Bj-BPP-10c | Mus musculus | |
| 0.36 | - |
citrulline | - |
Mus musculus | |
| 0.61 | - |
ATP | in presence of 0.1 mM Bj-BPP-10c | Mus musculus | |
| 0.66 | - |
ATP | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | P16460 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Homo sapiens |
| affinity chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293 cell | - |
Homo sapiens | - |
| HUVEC cell | - |
Homo sapiens | - |
| kidney | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + citrulline + aspartate | assay at pH 7.8, 37°C, reaction stopped by addition of molybdate buffer | Mus musculus | AMP + diphosphate + 2-(Nomega-L-arginino)succinate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Argininosuccinate synthetase | - |
Homo sapiens |
| Argininosuccinate synthetase | - |
Mus musculus |
| ASS | - |
Homo sapiens |
| ASS | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | part of citrulline-NO cycle, limiting step in NO synthesis | Homo sapiens |
| physiological function | part of citrulline-NO cycle, limiting step in NO synthesis | Mus musculus |
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Release 2023.1 (January 2023)
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