Literature summary for 6.3.4.5 extracted from

Endo, H.; Ootsuka, S.; Fukuda, S.; Kitade, Y.; Saga, N.
Functional complementation of an arginine auxotrophic yeast mutant by an argininosuccinate synthetase from Porphyra yezoensis (Rhodophyta) (2006), J. Phycol., 42, 1066-1071.

No PubMed abstract available

Application

Application	Comment	Organism
molecular biology	report in which the function of a Porphyra yezoensis gene has been directly demonstrated by the rescue of a Saccharomyces cerevisiae mutant. This technique may provide new opportunities for further investigations into the functions of various genes in Porphyra yezoensis and other macroalgal species	Neopyropia yezoensis

Cloned(Commentary)

Cloned (Comment)	Organism
the gene encoding argininosuccinate synthetase of Porphyra yezoensis is obtained by PCR using an expressed sequence tag clone as a template, and subcloned into the yeast expression vector pYES2. The gene is expressed when the vector harboring PyARG1 is introduced into an ARG1-deficient strain of Saccharomyces cerevisiae, which results in complementation of the mutant phenotype. The transformed cells survive on a selective medium lacking arginine, and transcripts of PyARG1 are detected by RT-PCR. A quantitative comparison shows that the rescued mutant cells grow in the selective liquid medium with a minor reduction in growth rate relative to wild-type cells	Neopyropia yezoensis

Cloned (Comment)

Organism

the gene encoding argininosuccinate synthetase of Porphyra yezoensis is obtained by PCR using an expressed sequence tag clone as a template, and subcloned into the yeast expression vector pYES2. The gene is expressed when the vector harboring PyARG1 is introduced into an ARG1-deficient strain of Saccharomyces cerevisiae, which results in complementation of the mutant phenotype. The transformed cells survive on a selective medium lacking arginine, and transcripts of PyARG1 are detected by RT-PCR. A quantitative comparison shows that the rescued mutant cells grow in the selective liquid medium with a minor reduction in growth rate relative to wild-type cells

Neopyropia yezoensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48500	-	deduced from cDNA	Neopyropia yezoensis

Organism

Organism	UniProt	Comment	Textmining
Neopyropia yezoensis	Q0KKQ0	-	-

Synonyms

Synonyms	Comment	Organism
Argininosuccinate synthetase	-	Neopyropia yezoensis
PyARG1	-	Neopyropia yezoensis

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Release 2023.1 (January 2023)