Literature summary for 6.3.4.5 extracted from

Hao, G.; Xie, L.; Gross, S.S.
Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo (2004), J. Biol. Chem., 279, 36192-36200.

Search for more literature for 6.3.4.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a C-terminal fusion protein with maltose-binding protein in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
NO	mediates reversible S-nitrosylation and inactivation of argininosuccinate synthetase in vitro and in lipopolysaccharide-treated cells and mice. S-nitrosylation of Cys132 is noth necessary and sufficient for the inhibition of argininosuccinate synthase by NO donors	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-citrulline + L-Asp	Homo sapiens	the substrate for NO synthesis, L-arginine can be regenerated from the L-citrulline the coproduct of nitric-oxide synthase. This requires the sequential action of two enzymes, argininosuccinate synthetase and argininosuccinate lyase	AMP + diphosphate + L-argininosuccinate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-citrulline + L-Asp	the substrate for NO synthesis, L-arginine can be regenerated from the L-citrulline the coproduct of nitric-oxide synthase. This requires the sequential action of two enzymes, argininosuccinate synthetase and argininosuccinate lyase	Homo sapiens	AMP + diphosphate + L-argininosuccinate	-	?

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Release 2023.1 (January 2023)