Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenylosuccinate | - |
Mus musculus | |
AMP | inhibits the acidic isozyme competitively, weak inhibition of the basic isozyme noncompetitively | Mus musculus | |
D-fructose 1,6-bisphosphate | inhibits both isozymes competitively | Mus musculus | |
GDP | - |
Mus musculus | |
GMP | - |
Mus musculus | |
IMP | competitive inhibition of the acidic isozyme, noncompetitive of the basic isozyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
GTP | pH 6.7, 22°C, AdSS1-Tr | Mus musculus | |
0.009 | - |
IMP | pH 6.7, 22°C, AdSS2-Tr | Mus musculus | |
0.012 | - |
GTP | pH 6.7, 22°C, AdSS1 | Mus musculus | |
0.012 | - |
IMP | pH 6.7, 22°C, AdSS2 | Mus musculus | |
0.013 | - |
GTP | pH 6.7, 22°C, AdSS2-Tr | Mus musculus | |
0.015 | - |
GTP | pH 6.7, 22°C, AdSS2 | Mus musculus | |
0.043 | - |
IMP | pH 6.7, 22°C, AdSS1-Tr | Mus musculus | |
0.045 | - |
IMP | pH 6.7, 22°C, AdSS1 | Mus musculus | |
0.14 | - |
L-aspartate | pH 6.7, 22°C, AdSS1 | Mus musculus | |
0.15 | - |
L-aspartate | pH 6.7, 22°C, AdSS1-Tr | Mus musculus | |
0.95 | - |
L-aspartate | pH 6.7, 22°C, AdSS2 | Mus musculus | |
1.03 | - |
L-aspartate | pH 6.7, 22°C, AdSS2-Tr | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | AdSS2 requires 2.0 mM Mg(acetate)2, AdSS1 requires 8.0 mM Mg(acetate)2 | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE, recombinant enzyme | Mus musculus |
86000 | - |
equilibrium sedimentation ultracentrifugation, AdSS1 | Mus musculus |
90700 | - |
equilibrium sedimentation ultracentrifugation, AdSS2 | Mus musculus |
100700 | - |
predicted from amino acid sequence, AdSS2 | Mus musculus |
101100 | - |
predicted from amino acid sequence, AdSS1 | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | Mus musculus | acidic and basic isozymes, which participates in the first committed step of de novo AMP biosynthesis and/or the purine nucleotide cycle | GDP + phosphate + adenylosuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
mouse | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | - |
Mus musculus | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | acidic and basic isozymes, which participates in the first committed step of de novo AMP biosynthesis and/or the purine nucleotide cycle | Mus musculus | GDP + phosphate + adenylosuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE, recombinant enzyme | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
Adenylosuccinate synthetase | - |
Mus musculus |
AdSS1 | basic isozyme | Mus musculus |
AdSS2 | acidic isozyme | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
GTP | pH 6.7, 22°C, AdSS1-Tr | Mus musculus | |
4 | - |
GTP | pH 6.7, 22°C, AdSS2-Tr | Mus musculus | |
4.2 | - |
GTP | pH 6.7, 22°C, AdSS2 | Mus musculus | |
5.4 | - |
GTP | pH 6.7, 22°C, AdSS1 | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
GMP | pH 6.7, 22°C, AdSS1 | Mus musculus | |
0.014 | - |
GMP | pH 6.7, 22°C, AdSS2 | Mus musculus | |
0.016 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS1, noncompetitive inhibition relative to IMP | Mus musculus | |
0.016 | - |
adenylosuccinate | pH 6.7, 22°C, AdSS2 | Mus musculus | |
0.019 | - |
GDP | pH 6.7, 22°C, AdSS1 | Mus musculus | |
0.019 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS2, noncompetitive inhibition relative to IMP | Mus musculus | |
0.021 | - |
adenylosuccinate | pH 6.7, 22°C, AdSS1 | Mus musculus | |
0.03 | - |
GDP | pH 6.7, 22°C, AdSS2 | Mus musculus | |
0.046 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS1 | Mus musculus | |
0.059 | - |
AMP | pH 6.7, 22°C, AdSS2 | Mus musculus | |
0.063 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS1, noncompetitive inhibition relative to GTP | Mus musculus | |
0.081 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS1, noncompetitive inhibition relative to L-aspartate | Mus musculus | |
0.128 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS2 | Mus musculus | |
0.4 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS2, noncompetitive inhibition relative to GTP | Mus musculus | |
0.67 | - |
D-fructose 1,6-bisphosphate | pH 6.7, 22°C, AdSS2, noncompetitive inhibition relative to L-aspartate | Mus musculus | |
0.7 | - |
AMP | pH 6.7, 22°C, AdSS1 | Mus musculus |