Literature summary for 6.3.4.4 extracted from

Liu, F.; Dong, Q.; Fromm, H.J.
Site-directed mutagenesis of the phosphate-binding consensus sequence in Escherichia coli adenylosuccinate synthetase (1992), J. Biol. Chem., 267, 2388-2392.

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Protein Variants

Protein Variants	Comment	Organism
G12V	replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme	Escherichia coli
G17V	replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme	Escherichia coli
L18R	replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	wild-type and mutant enzymes	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + IMP + L-Asp	-	Escherichia coli	GDP + phosphate + adenylosuccinate	-	?

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Release 2023.1 (January 2023)