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Literature summary for 6.3.4.4 extracted from
- Purification and properties of adenylosuccinate synthetase from Yoshida sarcoma ascites tumor cells (1980), Biochim. Biophys. Acta, 616, 340-350.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Rattus norvegicus |  |
| AMP | - |
Rattus norvegicus | |
| Ca2+ | in presence of Mg2+ | Rattus norvegicus | |
| Cd2+ | in presence of Mg2+ | Rattus norvegicus | |
| Cu2+ | in presence of Mg2+ | Rattus norvegicus | |
| dGTP | - |
Rattus norvegicus | |
| GDP | - |
Rattus norvegicus | |
| GMP | - |
Rattus norvegicus | |
| guanosine | - |
Rattus norvegicus | |
| Hadacidin | - |
Rattus norvegicus | |
| Hg2+ | reversed by addition of DTT | Rattus norvegicus | |
| IDP | - |
Rattus norvegicus | |
| Mn2+ | in presence of Mg2+ | Rattus norvegicus | |
| Pb2+ | in presence of Mg2+ | Rattus norvegicus | |
| PCMB | - |
Rattus norvegicus | |
| XMP | - |
Rattus norvegicus | |
| Zn2+ | in presence of Mg2+ | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
GTP | - |
Rattus norvegicus | |
| 0.41 | - |
IMP | - |
Rattus norvegicus | |
| 0.98 | - |
L-Asp | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 14.1% of the activation relative to Mg2+ | Rattus norvegicus | |
| Co2+ | 34.8% of the activation relative to Mg2+ | Rattus norvegicus | |
| Cu2+ | 10.9% of the activation relative to Mg2+ | Rattus norvegicus | |
| Mg2+ | full activity in presence of Mg2+ | Rattus norvegicus | |
| Mn2+ | 37% of the activation relative to Mg2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000, sedimentation equilibrium analysis in presence of 6 M guanidine-HCl | Rattus norvegicus |
| 102000 | - |
Yoshida sarcoma ascites tumor cells, sedimentation equilibrium analysis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Yoshida ascites sarcoma cell | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.36 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, or 0°C, rapid loss of enzyme activity in dilute solution | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + IMP + L-Asp | - |
Rattus norvegicus | GDP + phosphate + adenylosuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000, sedimentation equilibrium analysis in presence of 6 M guanidine-HCl | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7 | - |
Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
most stable at pH 7.0 in phosphate buffer | Rattus norvegicus |
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