Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Methanocaldococcus jannaschii | |
ADP | - |
Methanocaldococcus jannaschii | |
additional information | phosphonoacetaldehyde (20 mM) is not an inhibitor or substrate for the reaction | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ is the best divalent metal in supporting the reaction, with Mn2+ being 87% as active as Mg2+ | Methanocaldococcus jannaschii | |
Mn2+ | Mg2+ is the best divalent metal in supporting the reaction, with Mn2+ being 87% as active as Mg2+ | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Methanocaldococcus jannaschii | catalyses a step in the synthesis of purine nucleotides | ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57600 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.9 | - |
pH 7.3, 70°C | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | catalyses a step in the synthesis of purine nucleotides | Methanocaldococcus jannaschii | ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | no other nucleotide, when substituted for ATP, can function as a substrate | Methanocaldococcus jannaschii | ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
formylphosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Methanocaldococcus jannaschii | 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ? | - |
? | |
additional information | phosphonoacetaldehyde (20 mM) is not an inhibitor or substrate for the reaction | Methanocaldococcus jannaschii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-formaminoimidazole-4-carboxamide ribonucleotide synthetase | - |
Methanocaldococcus jannaschii |
5-formaminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate synthetase | - |
Methanocaldococcus jannaschii |
PurP | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the enzyme maintains 100% of activity when heated for 15 min at temperatures up to 100°C, but it loses all activity when heated above 100°C for 15 min | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Methanocaldococcus jannaschii |
7.3 | - |
assay at | Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity | Methanocaldococcus jannaschii |