Protein Variants | Comment | Organism |
---|---|---|
C17A | complete loss of activity | Archaeoglobus fulgidus |
C17S | complete loss of activity | Archaeoglobus fulgidus |
C210S | complete loss of activity | Archaeoglobus fulgidus |
D11A | complete loss of activity | Archaeoglobus fulgidus |
D8A | complete loss of activity | Archaeoglobus fulgidus |
D9A | complete loss of activity | Archaeoglobus fulgidus |
E159A | complete loss of activity | Archaeoglobus fulgidus |
F286A | complete loss of activity | Archaeoglobus fulgidus |
F301A | complete loss of activity | Archaeoglobus fulgidus |
I143A | reduced activity | Archaeoglobus fulgidus |
I49A | reduced activity | Archaeoglobus fulgidus |
K52A | complete loss of activity | Archaeoglobus fulgidus |
N194A | reduced activity | Archaeoglobus fulgidus |
N56A | complete loss of activity | Archaeoglobus fulgidus |
R217A | complete loss of activity | Archaeoglobus fulgidus |
R369A | complete loss of activity | Archaeoglobus fulgidus |
R44A | complete loss of activity | Archaeoglobus fulgidus |
R54A | complete loss of activity | Archaeoglobus fulgidus |
T18A | complete loss of activity | Archaeoglobus fulgidus |
T18D | complete loss of activity | Archaeoglobus fulgidus |
T53A | complete loss of activity | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,beta-methyleneadenosine 5'-triphosphate | - |
Archaeoglobus fulgidus | |
beta,gamma-methyleneadenosine 5'-triphosphate | - |
Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
[tRNAIle2]-cytidine34 | pH 8.8, 70°C | Archaeoglobus fulgidus | |
0.00087 | - |
agmatine | pH 8.8, 70°C | Archaeoglobus fulgidus | |
0.006 | - |
ATP | pH 8.8, 70°C | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal concentration: 100 mM | Archaeoglobus fulgidus | |
Mg2+ | optimal concentration: 2-5 mM | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + agmatine + [tRNAIle2]-cytidine34 | Archaeoglobus fulgidus | the enzyme catalyzes the essential modification at the anticodon wobble position (position 34) of the AUA-codon of tRNAIle | [tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28025 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the Thr18-Cyt34 kinase domain autophosphorylates the Thr18 of the enzyme | Archaeoglobus fulgidus |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + agmatine + [tRNAIle2]-cytidine34 | the enzyme catalyzes the essential modification at the anticodon wobble position (position 34) of the AUA-codon of tRNAIle | Archaeoglobus fulgidus | [tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate | - |
? | |
ATP + agmatine + [tRNAIle2]-cytidine34 | the precursor form of tRNAIle bearing an unmodified cytidine at the wobble position (C34) is aminoacylated with methionine (Met) and decodes the AUG codon.The Thr18-Cyt34 kinase domain first hydrolyzes ATP into AMP and diphosphate, then phosphorylates the C2 position of cytidine34 with the gamma-phosphate. Next, the amino group of agmatine attacks this position to release the phosphate and form 2-agmatinylcytidine. The Thr18-Cyt34 kinase domain also autophosphorylates the Thr18 of the enzyme, which may be involved in 2-agmatinylcytidine formation. Catalytic efficiency (kcat/Km) with GTP, UTP or CTP as substrate is less than 1% compared to ATP | Archaeoglobus fulgidus | [tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TiaS | - |
Archaeoglobus fulgidus |
tRNAIle-agm2C synthetase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
agmatine | pH 8.8, 70°C | Archaeoglobus fulgidus | |
0.0062 | - |
ATP | pH 8.8, 70°C | Archaeoglobus fulgidus | |
0.0065 | - |
[tRNAIle2]-cytidine34 | pH 8.8, 70°C | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Archaeoglobus fulgidus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00037 | - |
pH 8.8, 70°C | Archaeoglobus fulgidus | alpha,beta-methyleneadenosine 5'-triphosphate | |
0.00079 | - |
pH 8.8, 70°C | Archaeoglobus fulgidus | beta,gamma-methyleneadenosine 5'-triphosphate |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ATP | pH 8.8, 70°C | Archaeoglobus fulgidus | |
6.9 | - |
agmatine | pH 8.8, 70°C | Archaeoglobus fulgidus | |
22.41 | - |
[tRNAIle2]-cytidine34 | pH 8.8, 70°C | Archaeoglobus fulgidus |