Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | functional modulation by ATP via autophosphorylation of His232, activation by ATP hydrolysis, determination of the binding site | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of N-terminally tagged enzyme as selenomethionine-labeled variant in Escherichia coli strain DL41 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
recombinant selenomethionine-labeled enzyme, 10-30 mg/ml, in 10 mM Tris, pH 7.8, 150 mM NaCl, nanodroplet vapour diffusion method, 4°C, reservoir solution contains 6-16% PEG 5000 monomethyl ether, 0.06 M MES, and 0.04 M NaMES, pH 6.0, usage of 15-20% ethylene glycol as cryoprotectant, X-ray structure determination and analysis at 1.75 A resolution | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | molecular evolution mechanism | ? | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | Saccharomyces cerevisiae | essential for the NAD salvage pathway | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P39683 | gene NPT1 at ocus YOR209C, eukaryotic type II enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally tagged selenomethionine-labeled enzyme variant from Escherichia coli strain DL41 | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | active site structure | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | molecular evolution mechanism | Saccharomyces cerevisiae | ? | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Saccharomyces cerevisiae | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | essential for the NAD salvage pathway | Saccharomyces cerevisiae | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme is a member of the phosphoribosyltransferase superfamily or PRTase superfamily | Saccharomyces cerevisiae |
NAPRTase | - |
Saccharomyces cerevisiae |
nicotinic acid phosphoribosyltransferase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae |