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Literature summary for 6.3.4.21 extracted from

  • Chappie, J.S.; Canaves, J.M.; Han, G.W.; Rife, C.L.; Xu, Q.; Stevens, R.C.
    The structure of a eukaryotic nicotinic acid phosphoribosyltransferase reveals structural heterogeneity among type II PRTases (2005), Structure, 13, 1385-1396.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP functional modulation by ATP via autophosphorylation of His232, activation by ATP hydrolysis, determination of the binding site Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally tagged enzyme as selenomethionine-labeled variant in Escherichia coli strain DL41 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant selenomethionine-labeled enzyme, 10-30 mg/ml, in 10 mM Tris, pH 7.8, 150 mM NaCl, nanodroplet vapour diffusion method, 4°C, reservoir solution contains 6-16% PEG 5000 monomethyl ether, 0.06 M MES, and 0.04 M NaMES, pH 6.0, usage of 15-20% ethylene glycol as cryoprotectant, X-ray structure determination and analysis at 1.75 A resolution Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae molecular evolution mechanism ?
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Saccharomyces cerevisiae essential for the NAD salvage pathway beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P39683 gene NPT1 at ocus YOR209C, eukaryotic type II enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally tagged selenomethionine-labeled enzyme variant from Escherichia coli strain DL41 Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate active site structure Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information molecular evolution mechanism Saccharomyces cerevisiae ?
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
Saccharomyces cerevisiae beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O essential for the NAD salvage pathway Saccharomyces cerevisiae beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
r

Synonyms

Synonyms Comment Organism
More enzyme is a member of the phosphoribosyltransferase superfamily or PRTase superfamily Saccharomyces cerevisiae
NAPRTase
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Saccharomyces cerevisiae
nicotinic acid phosphoribosyltransferase
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Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Saccharomyces cerevisiae