General Stability | Organism |
---|---|
first order activity loss in the presence of 1/200 trypsin/enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
rapidly inactivates the ATPase and nicotinic acid mononucleotide synthesis activities with cleavages at Arg-384 and Lys-374, ATP and 5-phospho-alpha-D-ribose 1-diphosphate increase the t1/2 for inactivation 8fold and 4fold, respectively, and 32fold, together | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
bacterial suspension disruption and centrifugation, chromatography on Q-Sepharose and phenyl-Sepharose | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4ΒΊC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAPRTase | - |
- |
nicotinic acid phosphoribosyltransferase | - |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | 2000fold increase in the Kcat/KM | Salmonella enterica subsp. enterica serovar Typhimurium |