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Literature summary for 6.3.4.21 extracted from

  • Rajavel, M.; Gross, J.W.; Segura, E.; Moore, W.T.; Grubmeyer, C.
    Limited proteolysis of Salmonella typhimurium nicotinic acid phosphoribosyltransferase reveals ATP-linked conformational change (1996), Biochemistry, 35, 3909-3916.
    View publication on PubMed

General Stability

General Stability Organism
first order activity loss in the presence of 1/200 trypsin/enzyme Salmonella enterica subsp. enterica serovar Typhimurium
rapidly inactivates the ATPase and nicotinic acid mononucleotide synthesis activities with cleavages at Arg-384 and Lys-374, ATP and 5-phospho-alpha-D-ribose 1-diphosphate increase the t1/2 for inactivation 8fold and 4fold, respectively, and 32fold, together Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
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Purification (Commentary)

Purification (Comment) Organism
bacterial suspension disruption and centrifugation, chromatography on Q-Sepharose and phenyl-Sepharose Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4ΒΊC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme Salmonella enterica subsp. enterica serovar Typhimurium beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
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Synonyms

Synonyms Comment Organism
NAPRTase
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nicotinic acid phosphoribosyltransferase
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Cofactor

Cofactor Comment Organism Structure
ATP 2000fold increase in the Kcat/KM Salmonella enterica subsp. enterica serovar Typhimurium