Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | activates glutamine-dependent CTP formation at concentrations below 0.2 mM | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Caffeine | - |
Trypanosoma brucei | |
GTP | inhibits glutamine-dependent CTP formation at concentrations above 0.2 mM | Trypanosoma brucei | |
guanosine | - |
Trypanosoma brucei | |
uric acid | - |
Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + L-glutamine | Trypanosoma brucei | - |
ADP + phosphate + CTP + L-glutamate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-IDA resin column chromatography | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + L-glutamine | - |
Trypanosoma brucei | ADP + phosphate + CTP + L-glutamate | - |
ir | |
additional information | the enzyme can utilize NH3 as a substrate | Trypanosoma brucei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
CTP synthase | - |
Trypanosoma brucei |
CTPS | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.1 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | uric acid | |
0.38 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | guanosine | |
0.46 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | GTP | |
0.48 | - |
in HEPES buffer (70 mM, pH 7.3), at 37°C | Trypanosoma brucei | Caffeine |