Cloned (Comment) | Organism |
---|---|
protein is expressed as a His6-tagged wild type human CTP synthetase 1 fusion protein in Escherichia coli | Homo sapiens |
protein is expressed as a His6-tagged wild type human CTP synthetase 1 fusion protein in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S462A/T455A | S462A and T455A mutations result in a decreased CTP synthetase 1 phosphorylation which appear to be much less than of the individual mutant enzymes S462A or T455A | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
ATP | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | assay with 10 mM MgCl2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | CTP synthetase is phosphorylated by protein kinase C which leads to 95fold higher activity of CTP synthetase 1. Phosphopeptide mapping and phosphoamino acid analyses shows that CTP synthetase 1 is phosphorylated on multiple serine and threonine residues. It is indicated that protein kinase C phosphorylation at Ser462 stimulates human CTP synthetase 1 activity, whereas phosphorylation at Thr455 inhibits activity | Homo sapiens |
Purification (Comment) | Organism |
---|---|
His6-tagged wild type and mutant human CTP synthetase 1 enzymes are expressed and purified from yeast cell extracts with Ni2+-NTA resin | Homo sapiens |
His6-tagged wild type human CTP synthetase 1 is expressed and purified from Escherichia coli by Ni2+-NTA chromatography and with PorosHQ-ion-exchange chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
S462A mutation results in 61%-reduced CTP synthetase 1 phosphorylation. The Saccharomyces cerevisiae-expressed and purified S462A mutant enzyme exhibits a 2fold reduction in CTP synthetase 1 activity, whereas the purified T455A mutant enzyme exhibited a 2fold elevation in CTP synthetase 1 activity, implying that that protein kinase C phosphorylation at Ser462 stimulates human CTP synthetase 1 activity, whereas phosphorylation at Thr455 inhibits activity | Homo sapiens |
additional information | - |
T455A mutation results in 58%-reduced CTP synthetase 1 phosphorylation. The Saccharomyces cerevisiae-expressed and purified S462A mutant enzyme exhibits a 2fold reduction in CTP synthetase 1 activity, whereas the purified T455A mutant enzyme exhibited a 2fold elevation in CTP synthetase 1 activity, implying that that protein kinase C phosphorylation at Ser462 stimulates human CTP synthetase 1 activity, whereas phosphorylation at Thr455 inhibits activity | Homo sapiens |
0.0025 | - |
specific activity of Escherichia coli expressed enzyme, after a 20 min incubation with protein kinase C, the activity of the CTP synthetase was stimulated 95fold | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + Gln + H2O | - |
Homo sapiens | ADP + CTP + Glu + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CTP synthetase 1 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |