Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | required as allosteric effector to promote reaction with glutamine as substrate, GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression as a hexahistidine-tagged form | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C379A | mutant enzyme is fully active with ammonia but has no glutamine-dependent activity, no inhibition by glutamate gamma-semialdehyde | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D,L-2-amino-4-phosphonobutyrate | - |
Escherichia coli | |
DELTA1-Pyrroline-5-carboxylate | weak | Escherichia coli | |
glutamate gamma-semialdehyde | potent linear mixed-type inhibitor, competitive with respect to ammonia, no inhibition of the mutant enzyme C379A | Escherichia coli | |
L-2-pyrrolidone 5-carboxylate | weak competitive inhibition of the reaction with ammonia as substrate, no significant inhibition with glutamine as substrate | Escherichia coli | |
additional information | enzyme loses activity at ionic strengths higher than 0.4 M | Escherichia coli | |
pyrrole-2-carboxylate | weak competitive inhibition of the reaction with ammonia as substrate, no significant inhibition with glutamine as substrate | Escherichia coli | |
SO42- | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + glutamine | - |
Escherichia coli | ADP + phosphate + CTP + Glu | - |
? | |
ATP + UTP + NH4+ | - |
Escherichia coli | ADP + phosphate + CTP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
glutamine | pH 8.0, wild-type enzyme | Escherichia coli | |
8.1 | - |
glutamine | pH 8.0, recombinant His6-tagged enzyme | Escherichia coli | |
9.9 | - |
NH3 | pH 8.0, mutant enzyme C379A | Escherichia coli | |
10.4 | - |
NH3 | pH 8.0, mutant enzyme C379S | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.39 | - |
glutamate gamma-semialdehyde | - |
Escherichia coli | |
5.6 | - |
D,L-2-amino-4-phosphonobutyrate | pH 8.0, reaction with ammonia | Escherichia coli | |
11 | - |
pyrrole-2-carboxylate | pH 8.0, reaction with ammonia | Escherichia coli | |
12.6 | - |
L-2-pyrrolidone-5-carboxylate | pH 8.0, reaction with ammonia | Escherichia coli |