Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-acetylglutamate | the enzyme requires binding of and allosteric activation by N-acetylglutamate for catalytic activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene CPSI, expression of the N-terminally His6-FLAG-tagged enzyme in Schizosaccharomyces pombe from pESP5 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C1327A | the mutant shows reduced activity compared to the wild-type enzyme, the mutation significantly alters activity at the domain C ATP site, binding of N-acetylglutamate is affected, overview | Homo sapiens |
C1337A | the mutant shows reduced activity compared to the wild-type enzyme, the mutation significantly alters activity at the domain C ATP site, binding of N-acetylglutamate is affected, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview. The Km of the enzyme for ammonia is 100fold lower than that for CPSs that also use glutamine, EC 6.3.5.5 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial matrix | - |
Homo sapiens | 5759 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NH4+ + HCO3- + H2O | Homo sapiens | - |
ADP + phosphate + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P31327 | gene CPSI | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.43 | - |
purified recombinant tagged mutant C1327A | Homo sapiens |
0.47 | - |
purified recombinant tagged mutant C1337A | Homo sapiens |
0.86 | - |
purified recombinant tagged wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NH4+ + HCO3- + H2O | - |
Homo sapiens | ADP + phosphate + carbamoyl phosphate | - |
? | |
ATP + NH4+ + HCO3- + H2O | synthesis of carbamoyl phosphate requires coordination of two hCPS active sites with ATP cleavage occurring at duplicated ATP grasp domains B and C | Homo sapiens | ADP + phosphate + carbamoyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Carbamoyl-phosphate synthetase I | - |
Homo sapiens |
CPS1 | - |
Homo sapiens |
urea-specific CPS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |