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Literature summary for 6.3.4.15 extracted from
- Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation (2012), Protein Expr. Purif., 82, 162-167.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as thioredoxin- and maltose binding protein-fusion proteins in Escherichia coli BL21(DE3) cells | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli AVB101 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography, Ni-NTA resin column chromatography, and Superdex 75 gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + Avi-tagged thioredoxin | biotin ligase site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag) | Escherichia coli | ? | - |
? |  |
| ATP + biotin + Avi-tagged thioredoxin | biotin ligase site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag) | Escherichia coli AVB101 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * about 35000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| biotin ligase | - |
Escherichia coli |
| BirA | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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